TRXR1/TXNRD1 Recombinant Protein | TXNRD1 recombinant protein

TRXR1/TXNRD1 Protein, Human, Recombinant (aa 161-647, His Tag)

Cat. Num. AAA8121268

• Purity: >90% as determined by SDS-PAGE
• Synonyms: TRXR1/TXNRD1; TRXR1/TXNRD1 Protein; Human; Recombinant (aa 161-647; His Tag); Human TXNRD1/TRXR1 Protein (aa 161-647; GRIM-12 Protein; TR Protein; TR1 Protein; TRXR1 Protein; TXNR Protein; thioredoxin reductase 1; TXNRD1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Tyr161-Cys647

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human TXNRD1 isoform 1 (Q16881-1)(Tyr 161-Cys 647) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TXNRD1 recombinant protein

Background: Thioredoxin reductase 1 (TXNRD1) which is a selenocysteine-containing protein is overexpressed in many malignancies. TXNRD1 plays a key role in regulating cell growth and transformation, and protects cells against oxidative damage. We investigated the association between TXNRD1 polymorphisms and ATDH susceptibility. Moreover, TXNRD1 is an essential selenium-containing enzyme involved in detoxification of reactive oxygen species (ROS) and redox signaling. And genetic variations in TXNRD1 favor the development of Drug-induced liver injury (DILI), which is the most common adverse drug reaction.

References

Gasdaska P.Y., et al., 1995, FEBS Lett. 373:5-9. Rundloef A.-K., et al., 2004, Free Radic. Biol. Med. 36:641-656. Rikova K., et al., 2007, Cell 131:1190-1203. Fritz-Wolf K., et al., 2007, J. Mol. Biol. 370:116-127. Choudhary C., et al., 2009, Science 325:834-840.

NCBI and Uniprot Product Information

• NCBI GI #: 172046253
• NCBI GeneID: 7296
• UniProt Accession #: Q16881
• NCBI Official Full Name: Thioredoxin reductase 1, cytoplasmic
• UniProt Protein Name: Thioredoxin reductase 1, cytoplasmic
• Protein Family: Thioredoxin reductase
• UniProt Gene Name: TXNRD1
• UniProt Synonym Gene Names: GRIM12; KDRF; TR; GRIM-12; Gene associated with retinoic and IFN-induced mortality 12 protein
• UniProt Entry Name: TRXR1_HUMAN

NCBI Description

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

TRXR1: a cytoplasmic pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine residue which is required for the catalytic activity.

Protein type: Nucleotide Metabolism - pyrimidine; Nuclear receptor co-regulator; Oxidoreductase; EC 1.8.1.9

Chromosomal Location of Human Ortholog: 12q23-q24.1

Cellular Component: nucleoplasm; nucleolus; cytosol

Molecular Function: protein binding; thioredoxin-disulfide reductase activity; electron carrier activity; FAD binding; protein disulfide oxidoreductase activity

Biological Process: response to reactive oxygen species; nucleobase, nucleoside and nucleotide metabolic process; cell redox homeostasis; nucleobase, nucleoside and nucleotide interconversion; cellular lipid metabolic process; signal transduction