Western Blot (WB)
(ATPBD3 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the ATPBD3 antibody detected the ATPBD3 protein (arrow).)
Rabbit ATPBD3 Polyclonal Antibody | anti-CTU1 antibody
ATPBD3 Antibody (Center)
Gene Names
CTU1; NCS6; ATPBD3
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ATPBD3; Polyclonal Antibody; ATPBD3 Antibody (Center); Cytoplasmic tRNA 2-thiolation protein 1 {ECO:0000255|HAMAP-Rule:MF_03053}; 277- {ECO:0000255|HAMAP-Rule:MF_03053}; ATP-binding domain-containing protein 3 {ECO:0000255|HAMAP-Rule:MF_03053}; Cancer-associated gene protein; Cytoplasmic tRNA adenylyltransferase 1 {ECO:0000255|HAMAP-Rule:MF_03053}; CTU1 {ECO:0000255|HAMAP-Rule:MF_03053}; anti-CTU1 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ATPBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-236 amino acids from the Central region of human ATPBD3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
208-236
Sequence Length
348
Applicable Applications for anti-CTU1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(ATPBD3 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the ATPBD3 antibody detected the ATPBD3 protein (arrow).)
Western Blot (WB)
(ATPBD3 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the ATPBD3 antibody detected the ATPBD3 protein (arrow).)
Immunohistochemistry (IHC)
(ATPBD3 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATPBD3 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ATPBD3 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATPBD3 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-CTU1 antibody
References
Schlieker, C.D., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18255-18260(2008)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36450
NCBI Official Full Name
cytoplasmic tRNA 2-thiolation protein 1
NCBI Official Synonym Full Names
cytosolic thiouridylase subunit 1
NCBI Official Symbol
CTU1
NCBI Official Synonym Symbols
NCS6; ATPBD3
NCBI Protein Information
cytoplasmic tRNA 2-thiolation protein 1
UniProt Protein Name
Cytoplasmic tRNA 2-thiolation protein 1
Protein Family
UniProt Gene Name
CTU1
UniProt Entry Name
CTU1_HUMAN
Uniprot Description
ATPBD3: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Protein type: EC 2.7.7.-
Chromosomal Location of Human Ortholog: 19q13.41
Cellular Component: mitochondrion; cytosol
Molecular Function: protein binding; tRNA binding; nucleotidyltransferase activity
Biological Process: tRNA wobble uridine modification; protein urmylation
Research Articles on CTU1
Similar Products
Product Notes
The CTU1 ctu1 (Catalog #AAA9203996) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 208-236. The ATPBD3 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's ATPBD3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the CTU1 ctu1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATPBD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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