Rabbit anti-Human ATP7B Polyclonal Antibody

ATP7B, CT (Copper-transporting ATPase 2, Copper Pump 2, Wilson Disease-associated Protein, WND/140kD, WND, WC1, PWD)

Cat. Num. AAA624846

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: ATP7B; Polyclonal Antibody; CT (Copper-transporting ATPase 2; Copper Pump 2; Wilson Disease-associated Protein; WND/140kD; WND; WC1; PWD); Anti -ATP7B; anti-ATP7B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ATP7B.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-ATP7B antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human ATP7B (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATP7B antibody (C-term) in HepG2 cell line lysates (35ug/lane). ATP7B (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(ATP7B Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP7B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of ATP7B Antibody (C-term) with HepG2 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(ATP7B Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ATP7B antibody

ATP7B is a member of the P-type cation transport ATPase family and a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile.

Product Categories/Family for anti-ATP7B antibody

Antibodies; Abs to Enzymes; ATPase

NCBI and Uniprot Product Information

• NCBI GI #: 11037267
• NCBI Official Full Name: ATP7B

NCBI Description

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq, Jul 2008]

Uniprot Description

ATP7B: Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. Monomer. Interacts with COMMD1/MURR1. Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Transporter, ion channel; Membrane protein, integral; EC 3.6.3.54; Transporter; Vesicle; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: Golgi membrane; membrane; mitochondrion; basolateral plasma membrane; perinuclear region of cytoplasm; integral to plasma membrane; cytoplasmic membrane-bound vesicle; late endosome; trans-Golgi network

Molecular Function: protein binding; copper ion binding; copper-exporting ATPase activity; ATP binding

Biological Process: lactation; cellular copper ion homeostasis; response to copper ion; metabolic process; sequestering of calcium ion; cellular zinc ion homeostasis; copper ion import; copper ion transport; copper ion export; transmembrane transport; intracellular copper ion transport

Disease: Wilson Disease