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Western Blot (WB) (Western blot detection of ATP6V1E1 in 3T3,Hela,293 cell lysates using ATP6V1E1 Rabbit pAb(1:1000 diluted). Predicted band size:26KDa. Observed band size:26KDa.)

Rabbit ATP6V1E1 Polyclonal Antibody | anti-ATP6V1E1 antibody

ATP6V1E1 Rabbit pAb

Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Affinity purified
Synonyms
ATP6V1E1; Polyclonal Antibody; ATP6V1E1 Rabbit pAb; P31; Vma4; ATP6E; ARCL2C; ATP6E2; ATP6V1E; anti-ATP6V1E1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Ubiquitous (PubMed:12036578). High expression in the skin (PubMed:28065471).
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-ATP6V1E1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:1000
IHC: 1:50
ICC/IF: 1:20
FC/FACS: 1:20
IP: 1:20
Immunogen
Recombinant protein of human ATP6V1E1
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of ATP6V1E1 in 3T3,Hela,293 cell lysates using ATP6V1E1 Rabbit pAb(1:1000 diluted). Predicted band size:26KDa. Observed band size:26KDa.)

Western Blot (WB) (Western blot detection of ATP6V1E1 in 3T3,Hela,293 cell lysates using ATP6V1E1 Rabbit pAb(1:1000 diluted). Predicted band size:26KDa. Observed band size:26KDa.)
Related Product Information for anti-ATP6V1E1 antibody
Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

NCBI and Uniprot Product Information

NCBI GeneID
529
UniProt Accession #
Molecular Weight
Calculated MW: 26kDa
Observed MW: 26kDa
UniProt Protein Name
V-type proton ATPase subunit e 1
UniProt Gene Name
ATP6V0E1
UniProt Synonym Gene Names
ATP6H; ATP6V0E; V-ATPase subunit e 1
UniProt Entry Name
VA0E1_HUMAN

Uniprot Description

ATP6V0E1: Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase e1/e2 subunit family.

Protein type: Transporter, ion channel; Membrane protein, integral; Transporter, iron; Transporter; Hydrolase; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: phagocytic vesicle membrane; membrane; integral to membrane; endosome membrane

Molecular Function: ATPase activity, coupled to transmembrane movement of ions; transporter activity; hydrogen ion transporting ATPase activity, rotational mechanism

Biological Process: interaction with host; vacuolar acidification; proton transport; cellular iron ion homeostasis; metabolic process; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; response to amino acid stimulus; cell growth; transmembrane transport

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Product Notes

The ATP6V1E1 atp6v0e1 (Catalog #AAA4751687) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP6V1E1 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATP6V1E1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:1000 IHC: 1:50 ICC/IF: 1:20 FC/FACS: 1:20 IP: 1:20. Researchers should empirically determine the suitability of the ATP6V1E1 atp6v0e1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP6V1E1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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