Rabbit anti-Human ATP6V1C2 Polyclonal Antibody | anti-ATP6V1C2 antibody

ATP6V1C2, ID (ATP6V1C2, V-type proton ATPase subunit C 2, Vacuolar proton pump subunit C 2) (FITC)

Cat. Num. AAA6276692

• Gene Names: ATP6V1C2; VMA5; ATP6C2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATP6V1C2; Polyclonal Antibody; ID (ATP6V1C2; V-type proton ATPase subunit C 2; Vacuolar proton pump subunit C 2) (FITC); Vacuolar proton pump subunit C 2; anti-ATP6V1C2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 381

• Applicable Applications for anti-ATP6V1C2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ATP6V1C2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-304 amino acids from the Central region of human ATP6V1C2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ATP6V1C2 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the ATP6V1C2 antibody detected the ATP6V1C2 protein (arrow).)

Related Product Information for anti-ATP6V1C2 antibody

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A,three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain C subunit isoforms.

Product Categories/Family for anti-ATP6V1C2 antibody

Antibodies; Enzymes; ATPase

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Gruber, G. Biochem. Soc. Trans. 33 (PT 4), 883-885 (2005): Morel, N. Biol. Cell 95(7):453-457(2003). Smith, A.N., et al. Mol. Cell 12(4):801-803(2003). Kawasaki-Nishi, S., et al. FEBS Lett. 545(1):76-85(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 47717098
• NCBI GeneID: 245973
• NCBI Accession #: NP_653184.2
• NCBI GenBank Nucleotide #: NP_653184.2
• UniProt Accession #: Q8NEY4
• NCBI Official Full Name: V-type proton ATPase subunit C 2 isoform b
• NCBI Official Synonym Full Names: ATPase H+ transporting V1 subunit C2
• NCBI Official Symbol: ATP6V1C2
• NCBI Official Synonym Symbols: VMA5; ATP6C2
• NCBI Protein Information: V-type proton ATPase subunit C 2
• UniProt Protein Name: V-type proton ATPase subunit C 2
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V1C2
• UniProt Synonym Gene Names: V-ATPase subunit C 2
• UniProt Entry Name: VATC2_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A,three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain C subunit isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V1C2: Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase C subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Hydrolase

Chromosomal Location of Human Ortholog: -

Cellular Component: lysosomal membrane; cytosol

Molecular Function: hydrogen-exporting ATPase activity, phosphorylative mechanism; protein dimerization activity

Biological Process: interaction with host; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport; positive regulation of Wnt receptor signaling pathway