Rabbit anti-Human ATP6V0D1/VPATPD Polyclonal Antibody | anti-ATP6V0D1 antibody

Anti-ATP6V0D1/VPATPD Antibody, Rabbit Polyclonal

Cat. Num. AAA8103823

• Gene Names: ATP6V0D1; P39; VATX; VMA6; ATP6D; ATP6DV; VPATPD
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: ATP6V0D1/VPATPD; Polyclonal Antibody; Anti-ATP6V0D1/VPATPD Antibody; Rabbit Polyclonal; ATP6V0D1/VPATPD Antibody; Rabbit PAb; Antigen Affinity Purified; ATPase; H+ transporting; lysosomal 38kDa; V0 subunit d1; Anti-ATP6D Antibody; Anti-ATP6DV Antibody; Anti-P39 Antibody; Anti-VATX Antibody; Anti-VMA6 Antibody; Anti-VPATPD Antibody; anti-ATP6V0D1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ATP6V0D1/VPATPD
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-ATP6V0D1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Human ATP6V0D1/VPATPD
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ATP6V0D1/VPATPD, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ATP6V0D1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:40 kDa Observed band size:44 kDa (We are unsure as to the identity of these extra bands.))

Related Product Information for anti-ATP6V0D1 antibody

In the epididymis and vas deferens, the vacuolar H(+)ATPase (V-ATPase), located in the apical pole of narrow and clear cells, is required to establish an acidic luminal pH. Low pH is important for the maturation of sperm and their storage in a quiescent state. The V-ATPase also participates in the acidification of intracellular organelles. The V-ATPase contains many subunits, and several of these subunits have multiple isoforms. Subunits ATP6V0A2 and ATP6V1A were detected in intracellular structures closely associated but not identical to the TGN of principal cells and narrow/clear cells, and subunit ATP6V0D1 was strongly expressed in the apical membrane of principal cells in the apparent absence of other V-ATPase subunits. In principal cells, the ATP6V0D1 isoform may have a physiological function that is distinct from its role in proton transport via the V-ATPase complex. The agouti-related protein (AgRP) is an orexigenic peptide that plays a significant role in the regulation of energy balance. Using an elaborate in vitro screening approach, we show here that two adjacent enhancers inside the first intron of the neighboring (1.4 kb downstream) ATPase gene (ATP6V0D1) modulate the human AgRP promoter with profound spatiotemporal variation despite their diminutive sizes (221 and 231 nt). In transgenic mice, the proximal enhancer displayed specificity for the testis, tail, and ears, and the distal one for the testis, front feet, bone, heart, muscle, brain, spinal cord, and tongue, while dietary fat and overnight fasting had differential effects on enhancer activities. Spatiotemporal expression of the human AgRP gene is influenced by diversified primate-specific intronic sequences in its neighboring ATP6V0D1 gene.

NCBI and Uniprot Product Information

• NCBI GI #: 47606646
• NCBI GeneID: 9114
• UniProt Accession #: P61421; P12953; Q02547
• Molecular Weight: 351
• NCBI Official Full Name: V-type proton ATPase subunit d 1
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
• NCBI Official Symbol: ATP6V0D1
• NCBI Official Synonym Symbols: P39; VATX; VMA6; ATP6D; ATP6DV; VPATPD
• NCBI Protein Information: V-type proton ATPase subunit d 1; V-ATPase, subunit D; V-ATPase subunit d 1; V-ATPase AC39 subunit; 32 kDa accessory protein; vacuolar proton pump subunit d 1; V-ATPase 40 KDa accessory protein; H(+)-transporting two-sector ATPase, subunit D; ATPase, H+ t
• UniProt Protein Name: V-type proton ATPase subunit d 1
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V0D1
• UniProt Synonym Gene Names: ATP6D; VPATPD; V-ATPase subunit d 1; p39
• UniProt Entry Name: VA0D1_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V0D1: Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis. Belongs to the V-ATPase V0D/AC39 subunit family.

Protein type: Hydrolase; Vesicle; EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: centrosome; phagocytic vesicle membrane; synaptic vesicle; membrane; lysosomal membrane; apical plasma membrane; early endosome; endosome membrane; vacuolar proton-transporting V-type ATPase complex; nerve terminal

Molecular Function: hydrogen-exporting ATPase activity, phosphorylative mechanism; protein binding; protein complex binding

Biological Process: interaction with host; proton transport; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; unfolded protein response; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; cilium biogenesis; brain development; transmembrane transport