Rabbit anti-Human ATP6V0A2 Polyclonal Antibody | anti-ATP6V0A2 antibody

ATP6V0A2 Antibody

Cat. Num. AAA9602865

• Gene Names: ATP6V0A2; A2; RTF; TJ6; WSS; ARCL; J6B7; STV1; TJ6M; TJ6S; VPH1; ARCL2A; ATP6A2; ATP6N1D
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: ATP6V0A2; Polyclonal Antibody; ATP6V0A2 Antibody; a2; A2V ATPase; ARCL; ATP6a2; ATP6N1D; ATPase; H+ transporting; lysosomal V0 subunit a isoform 2; lysosomal V0 subunit a2; Infantile malignant osteopetrosis; J6B7; Lysosomal H(+) transporting ATPase V0 subunit a2; Lysosomal H(+)-transporting ATPase V0 subunit a2; regeneration and tolerance factor; Stv1; TJ6; TJ6M; TJ6s; V ATPase 116 kDa isoform a2; V type proton ATPase 116 kDa subunit a; V type proton ATPase 116 kDa subunit a isoform 2; V-ATPase 116 kDa isoform a2; V-type proton ATPase 116 kDa subunit a isoform 2; Vacuolar proton translocating ATPase 116 kDa subunit a; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2; Vph1; VPP2_HUMAN; WSS; anti-ATP6V0A2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATP6V0A2 antibody detects endogenous levels of total ATP6V0A2
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 856

• Applicable Applications for anti-ATP6V0A2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human ATP6V0A2
• Subcellular Location: Cell Membrane. Endosome Membrane. In kidney proximal tubules, also detected in subapical vesicles.
• Predicted Cross Reactivity: Bovine, Horse, Sheep, Dog
• Similarity: Bovine (100%), Horse (92%), Sheep (100%), Dog (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of ATP6V0A2 using COLO205 whole cell lysates)

Related Product Information for anti-ATP6V0A2 antibody

Function: Part of the proton channel of V-ATPases. Essential component of the endosomal pH-sensing machinery. May play a role in maintaining the Golgi functions, such as glycosylation maturation, by controlling the Golgi pH. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633).
Subunit Structure: The V-ATPase is a heteromultimeric enzyme composed of at least thirteen different subunits. It has a membrane peripheral V1 sector for ATP hydrolysis and an integral V0 for proton translocation. The V1 sector comprises subunits A-H, whereas V0 includes subunits a, d, c, c', and c''. Directly interacts with PSCD2 through its N-terminal cytosolic tail in an intra-endosomal acidification-dependent manner (PubMed:16415858). Disruption of this interaction results in the inhibition of endocytosis (PubMed:16415858). Interacts with SPAAR (PubMed:28024296).
Similarity: Belongs to the V-ATPase 116 kDa subunit family.

NCBI and Uniprot Product Information

• NCBI GI #: 42741679
• NCBI GeneID: 23545
• NCBI Accession #: NP_036595.2
• NCBI GenBank Nucleotide #: NM_012463.3
• UniProt Accession #: Q9Y487; Q6NUM0; A8K026
• Molecular Weight: Observed: 98 kDa; Predicted: 99 kDa
• NCBI Official Full Name: V-type proton ATPase 116 kDa subunit a isoform 2
• NCBI Official Synonym Full Names: ATPase H+ transporting V0 subunit a2
• NCBI Official Symbol: ATP6V0A2
• NCBI Official Synonym Symbols: A2; RTF; TJ6; WSS; ARCL; J6B7; STV1; TJ6M; TJ6S; VPH1; ARCL2A; ATP6A2; ATP6N1D
• NCBI Protein Information: V-type proton ATPase 116 kDa subunit a isoform 2; V-type proton ATPase 116 kDa subunit a
• UniProt Protein Name: V-type proton ATPase 116 kDa subunit a isoform 2
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V0A2
• UniProt Synonym Gene Names: V-ATPase 116 kDa isoform a2

NCBI Description

The protein encoded by this gene is a subunit of the vacuolar ATPase (v-ATPase), an heteromultimeric enzyme that is present in intracellular vesicles and in the plasma membrane of specialized cells, and which is essential for the acidification of diverse cellular components. V-ATPase is comprised of a membrane peripheral V(1) domain for ATP hydrolysis, and an integral membrane V(0) domain for proton translocation. The subunit encoded by this gene is a component of the V(0) domain. Mutations in this gene are a cause of both cutis laxa type II and wrinkly skin syndrome. [provided by RefSeq, Jul 2009]

Uniprot Description

Part of the proton channel of V-ATPases. Essential component of the endosomal pH-sensing machinery. May play a role in maintaining the Golgi functions, such as glycosylation maturation, by controlling the Golgi pH. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633).