Testing Data
(Confocal IF analysis of HeLa (Top Pane) and A431 (Bottom Pane) using MBS613593 (Red). Actin filaments have been labeled with Alexa Fluor 488 - Phalloidin (Green). Nucleus is stained with DAPI (Blue).)
Rabbit Brn3A Polyclonal Antibody | anti-Pou4f1 antibody
Brn3A (Pou Domain Transcription Factor)
Purified by affinity chromatography.
Purified by affinity chromatography.
Dilution: Western Blot: 1:500 detected Brn3A on 10ug of bovine and rat retina lysates.
Immunocytochemistry: 1:500 detected Brn3A in HeLa and A431 lysates.
Immunohistochemistry (Frozen): 1:200-1:1000 for tissue.
Testing Data
(Confocal IF analysis of HeLa (Top Pane) and A431 (Bottom Pane) using MBS613593 (Red). Actin filaments have been labeled with Alexa Fluor 488 - Phalloidin (Green). Nucleus is stained with DAPI (Blue).)
Testing Data
(Confocal IF analysis of HeLa (Top Pane) and A431 (Bottom Pane) using MBS613593 (Red). Actin filaments have been labeled with Alexa Fluor 488 - Phalloidin (Green). Nucleus is stained with DAPI (Blue).)
Western Blot (WB)
(Western Blot Analysis: Bovine Retina lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS613593 (1:500). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein Brn3a (~42kD).)
Western Blot (WB)
(Western Blot Analysis: Bovine Retina lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS613593 (1:500). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein Brn3a (~42kD).)
NCBI and Uniprot Product Information
Uniprot Description
POU4F1: Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons. Belongs to the POU transcription factor family. Class- 4 subfamily.
Protein type: DNA-binding; Cell development/differentiation; Transcription factor
Cellular Component: neuron projection; nuclear chromatin; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; nervous system development; central nervous system neuron differentiation; trigeminal nerve development; transcription, DNA-dependent; positive regulation of apoptosis; multicellular organismal development; peripheral nervous system neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; habenula development; regulation of transcription from RNA polymerase II promoter; cell migration in hindbrain; peripheral nervous system neuron development; neuron differentiation; proprioception during equilibrioception; regulation of neurogenesis; suckling behavior; regulation of transcription, DNA-dependent; neuron fate specification; mesoderm development; positive regulation of transcription from RNA polymerase II promoter; sensory system development; negative regulation of neuron apoptosis
Research Articles on Pou4f1
2. Data show that Brn3a acts upstream of the Runx factors, which then repress TrkB expression to allow establishment of the non-overlapping Trk receptor profiles and correct terminally differentiated phenotypes.
3. Brn3a regulates the transition from neurogenesis to terminal differentiation and represses non-neural gene expression in the trigeminal ganglion.
4. these findings begin to define a gene regulatory pathway for habenula development in mammals, and the roles of Nurr1 and Brn3a therein.
5. Brn-3a deficiency affects the number and cell size of CGRP- and calcium-binding protein-containing neurons.
6. Loss of Brn3a leads to an increase in the ratio of bistratified to monostratified RGCs, with only modest RGC loss and little effect on central projections.
7. Brn3a represses differences in gene expression between sensory neurons generated at different axial levels, and regulates developmental gene expression; functionally similar populations of neurons are generated from different embryological substrates.
8. results suggest that co-factors binding to p73 C terminus facilitate activation on Bax but not p21 CIP1/Waf1 promoter and that Brn-3a modulates this interaction
9. Data suggest that Brn-3a and b POU factors may play an important role in the development and maintenance of critical cell types and structures within the heart, in part via developmental regulation of myocardial Hsp27 expression.
10. Data show that Pou4f1 is regionally enriched in the Brainstem (Pons and Medulla) of mouse brain.
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Product Notes
The Pou4f1 pou4f1 (Catalog #AAA613593) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Brn3A (Pou Domain Transcription Factor) reacts with Bovine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Brn3A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Dilution: Western Blot: 1:500 detected Brn3A on 10ug of bovine and rat retina lysates. Immunocytochemistry: 1:500 detected Brn3A in HeLa and A431 lysates. Immunohistochemistry (Frozen): 1:200-1:1000 for tissue. Researchers should empirically determine the suitability of the Pou4f1 pou4f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Brn3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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