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Western Blot (WB) (Western blot analysis of the lysates from 293 cells using ATP5I antibody.)

Rabbit anti-Human ATP5I Polyclonal Antibody | anti-ATP5I antibody

ATP5I Antibody

Gene Names
ATP5ME; ATP5I; ATP5K
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
ATP5I; Polyclonal Antibody; ATP5I Antibody; ATP synthase e chain mitochondrial; ATP5K; F1F0-ATP synthase; anti-ATP5I antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
ATP5I Antibody detects endogenous levels of ATP5I.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
69
Applicable Applications for anti-ATP5I antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:10000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human ATP5I.
Immunogen Range
20-69
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Western Blot (WB)

(Western blot analysis of the lysates from 293 cells using ATP5I antibody.)

Western Blot (WB) (Western blot analysis of the lysates from 293 cells using ATP5I antibody.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
521
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
7 kDa
NCBI Official Full Name
ATP synthase subunit e, mitochondrial
NCBI Official Synonym Full Names
ATP synthase membrane subunit e
NCBI Official Symbol
ATP5ME
NCBI Official Synonym Symbols
ATP5I; ATP5K
NCBI Protein Information
ATP synthase subunit e, mitochondrial
UniProt Protein Name
ATP synthase subunit e, mitochondrial
Protein Family
UniProt Gene Name
ATP5I
UniProt Synonym Gene Names
ATP5K; ATPase subunit e

NCBI Description

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the e subunit of the Fo complex. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]

Uniprot Description

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane.

Research Articles on ATP5I

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Product Notes

The ATP5I atp5i (Catalog #AAA9509209) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP5I Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATP5I can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the ATP5I atp5i for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP5I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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