ATP5F1 recombinant protein

ATP5F1, 83-256aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205757

• Gene Names: ATP5PB; PIG47; ATP5F1
• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: ATP5F1; 83-256aa; Human; His tag; E Coli (Denatured); ATP synthase subunit b; PIG47; ATP5F1 recombinant protein

• Host: E Coli
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSLILYALS KEIYVISAET FTALSVLGVM VYGIKKYGPF VADFADKLNE QKLAQLEEAK QASIQHIQNA IDTEKSQQAL VQKRHYLFDV QRNNIAMALE VTYRERLYRV YKEVKNRLDY HISVQNMMRR KEQEHMINWV EKHVVQSIST QQEKETIAKC IADLKLLAKK AQAQPVM
• Sequence Length: 256

• Applicable Applications for ATP5F1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ATP5F1 recombinant protein

ATP5F1 is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). Recombinant human ATP5F1 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for ATP5F1 recombinant protein

Metabolism

References

Higuti T., et al. (1991) Biochem. Biophys. Res. Commun. 178:1014-1020; Choudhary C., et al. (2009) Science. 325:834-840;

NCBI and Uniprot Product Information

• NCBI GI #: 21361565
• NCBI GeneID: 515
• NCBI Accession #: NP_001679
• NCBI GenBank Nucleotide #: NM_001688.4
• UniProt Accession #: P24539; Q9BQ68; Q9BRU8
• Molecular Weight: 22.6 kDa (197aa)
• NCBI Official Full Name: ATP synthase F(0) complex subunit B1, mitochondrial
• NCBI Official Synonym Full Names: ATP synthase peripheral stalk-membrane subunit b
• NCBI Official Symbol: ATP5PB
• NCBI Official Synonym Symbols: PIG47; ATP5F1
• NCBI Protein Information: ATP synthase F(0) complex subunit B1, mitochondrial
• UniProt Protein Name: ATP synthase F(0) complex subunit B1, mitochondrial
• Protein Family: ATP synthase F(0) complex
• UniProt Gene Name: ATP5F1
• UniProt Synonym Gene Names: ATPase subunit b

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq, Jul 2008]

Uniprot Description

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements.