Rabbit anti-Human ATP5C1 Polyclonal Antibody | anti-ATP5C1 antibody

ATP5C1, NT (ATP5C1, ATP5C, ATP5CL1, ATP synthase subunit gamma, mitochondrial, F-ATPase gamma subunit) (FITC)

Cat. Num. AAA6276505

• Gene Names: ATP5C1; ATP5C; ATP5CL1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATP5C1; Polyclonal Antibody; NT (ATP5C1; ATP5C; ATP5CL1; ATP synthase subunit gamma; mitochondrial; F-ATPase gamma subunit) (FITC); F-ATPase gamma subunit; anti-ATP5C1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ATP5C1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ATP5C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46~76 amino acids from the N-terminal region of human ATP5C1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATP5C1 Antibody (N-term) in HL-60 cell line lysates (35ug/lane).ATP5C1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ATP5C1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ATP5C1 Antibody (N-term) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ATP5C1 antibody

Antibodies; Mitochondrial Proteins

References

Wheeler, H.E., et.al, PLoS Genet. 5 (10), E1000685 (2009). Wang, L., et.al, Cancer Epidemiol. Biomarkers Prev. 17 (12), 3558-3566 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 50345988
• NCBI GeneID: 509
• NCBI Accession #: NP_001001973.1
• NCBI GenBank Nucleotide #: NM_001001973.1
• UniProt Accession #: P36542; Q5VYP3; Q6I9V2; Q96AS8; A8KA31
• Molecular Weight: 32,996 Da
• NCBI Official Full Name: ATP synthase subunit gamma, mitochondrial isoform L (liver)
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
• NCBI Official Symbol: ATP5C1
• NCBI Official Synonym Symbols: ATP5C; ATP5CL1
• NCBI Protein Information: ATP synthase subunit gamma, mitochondrial; F-ATPase gamma subunit; ATP synthase gamma chain, mitochondrial; mitochondrial ATP synthase, gamma subunit 1
• UniProt Protein Name: ATP synthase subunit gamma, mitochondrial
• UniProt Gene Name: ATP5C1
• UniProt Synonym Gene Names: ATP5C; ATP5CL1
• UniProt Entry Name: ATPG_HUMAN

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP5C: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Belongs to the ATPase gamma chain family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Hydrolase; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: membrane; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; mitochondrial proton-transporting ATP synthase complex

Molecular Function: ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; transmembrane transporter activity; hydrogen ion transporting ATPase activity, rotational mechanism

Biological Process: cellular metabolic process; ATP biosynthetic process; mitochondrial ATP synthesis coupled proton transport; oxidative phosphorylation