Rabbit anti-Human ATP5C1 Polyclonal Antibody | anti-ATP5C1 antibody

ATP5C1, NT (ATP5C1, ATP5C, ATP5CL1, ATP synthase subunit gamma, mitochondrial, F-ATPase gamma subunit)

Cat. Num. AAA646125

• Gene Names: ATP5C1; ATP5C; ATP5CL1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ATP5C1; Polyclonal Antibody; NT (ATP5C1; ATP5C; ATP5CL1; ATP synthase subunit gamma; mitochondrial; F-ATPase gamma subunit); Anti -ATP5C1; anti-ATP5C1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ATP5C1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: ATP5C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46~76 amino acids from the N-terminal region of human ATP5C1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATP5C1 Antibody (N-term) in HL-60 cell line lysates (35ug/lane).ATP5C1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ATP5C1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Testing Data

(ATP5C1 Antibody (N-term) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ATP5C1 antibody

ATP5C1 encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3.

Product Categories/Family for anti-ATP5C1 antibody

Antibodies; Abs to Mitochondrial Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 48146923
• NCBI GeneID: 509
• UniProt Accession #: Q6I9V2; Q5VYP3; Q96AS8; A8KA31; P36542
• Molecular Weight: 32,996 Da
• NCBI Official Full Name: ATP5C1
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
• NCBI Official Symbol: ATP5C1
• NCBI Official Synonym Symbols: ATP5C; ATP5CL1
• NCBI Protein Information: ATP synthase subunit gamma, mitochondrial; F-ATPase gamma subunit; ATP synthase gamma chain, mitochondrial; mitochondrial ATP synthase, gamma subunit 1
• UniProt Protein Name: ATP synthase subunit gamma, mitochondrial
• UniProt Gene Name: ATP5C1
• UniProt Synonym Gene Names: ATP5C; ATP5CL1
• UniProt Entry Name: ATPG_HUMAN

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F1 domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha3beta3. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Subunit structure: F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a, b and c. Component of an ATP synthase complex composed of ATP5F1, ATP5G1, ATP5E, ATP5H, ATP5I, ATP5J, ATP5J2, MT-ATP6, MT-ATP8, ATP5A1, ATP5B, ATP5D, ATP5C1, ATP5O, ATP5L, USMG5 and MP68

By similarity.

Subcellular location: Mitochondrion. Mitochondrion inner membrane; Peripheral membrane protein

By similarity.

Tissue specificity: Isoform Heart is expressed specifically in the heart and skeletal muscle, which require rapid energy supply. Isoform Liver is expressed in the brain, liver and kidney. Isoform Heart and Isoform Liver are expressed in the skin, intestine, stomach and aorta.

Sequence similarities: Belongs to the ATPase gamma chain family.