Rabbit ATP2B2 Polyclonal Antibody | anti-ATP2B2 antibody
ATP2B2
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ATP2B2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
ATP2B2: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Hydrolase; Membrane protein, multi-pass; Transporter, ion channel; Transporter; Endoplasmic reticulum; Cell development/differentiation; EC 3.6.3.8
Chromosomal Location of Human Ortholog: 3p25.3
Cellular Component: cell soma; endoplasmic reticulum; cytoplasm; apical plasma membrane; plasma membrane; integral to membrane; synapse; cell junction; cilium
Molecular Function: calmodulin binding; protein C-terminus binding; protein binding; calcium-transporting ATPase activity; metal ion binding; calcium ion binding; calcium-dependent ATPase activity; ATP binding; PDZ domain binding
Biological Process: lactation; cytosolic calcium ion homeostasis; organelle organization and biogenesis; otolith mineralization; regulation of synaptic plasticity; locomotory behavior; detection of mechanical stimulus involved in sensory perception of sound; cerebellar granule cell differentiation; neuron differentiation; sensory perception of sound; transport; calcium ion transport; cerebellar Purkinje cell differentiation; synapse organization and biogenesis; auditory receptor cell stereocilium organization and biogenesis; blood coagulation; locomotion; neuromuscular process controlling balance; positive regulation of calcium ion transport; transmembrane transport; regulation of cell size; cGMP metabolic process; serotonin metabolic process
Disease: Deafness, Autosomal Recessive 12
