Rabbit ATP2A2 Polyclonal Antibody | anti-ATP2A2 antibody

ATP2A2 Polyclonal Antibody

Cat. Num. AAA3006485

• Gene Names: ATP2A2; DD; DAR; ATP2B; SERCA2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ATP2A2; Polyclonal Antibody; ATP2A2 Polyclonal Antibody; Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA2; SR Ca(2+)-ATPase 2; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type; slow twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; ATP2B; anti-ATP2A2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATP2A2 polyclonal antibody detects endogenous levels of ATP2A2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 997

• Applicable Applications for anti-ATP2A2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human ATP2A2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ATP2A2 polyclonal antibody at 1:500 dilutionLane1:THP-1 whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:PC12 whole cell lysate)

Related Product Information for anti-ATP2A2 antibody

ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The sarcoplasmic reticulum of striated muscle is a specialized intracellular membrane system that plays a critical role in the contraction and relaxation of muscle. The SERCAs mediate Ca2+ uptake into intracellular stores. SERCA-mediated Ca2+ uptake induces and maintains muscular relaxation. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue-dependent processing which is responsible for the generation of the SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle, and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells. SERCA2 plays an important part in regulating cardiac contractile function. SERCA3 is an isoform expressed in several cell types including platelets, lymphoid cells and mast cells. SERCA1, SERCA2 and SERCA3 all undergo alternative splicing.

NCBI and Uniprot Product Information

• NCBI GI #: 4502285
• NCBI GeneID: 488
• NCBI Accession #: NP_001672.1
• NCBI GenBank Nucleotide #: NP_001672.1
• UniProt Accession #: P16615
• Molecular Weight: ~ 110kDa
• NCBI Official Full Name: sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform a
• NCBI Official Synonym Full Names: ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
• NCBI Official Symbol: ATP2A2
• NCBI Official Synonym Symbols: DD; DAR; ATP2B; SERCA2
• NCBI Protein Information: sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• UniProt Protein Name: Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• Protein Family: Sarcoplasmic/endoplasmic reticulum calcium ATPase
• UniProt Gene Name: ATP2A2
• UniProt Synonym Gene Names: ATP2B; SERCA2
• UniProt Entry Name: AT2A2_HUMAN

NCBI Description

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of the skeletal muscle. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Other types of mutations in this gene have been associated with various forms of muscular dystrophies. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2019]