ATP1B1 recombinant protein

ATP1B1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121652

• Gene Names: ATP1B1; ATP1B
• Purity: >95% as determined by SDS-PAGE
• Synonyms: ATP1B1; ATP1B1 Protein; Human; Recombinant (His Tag); Human ATP1B1 Protein (His Tag); ATP1B Protein; ATPase; Na+/K+ transporting; beta 1 polypeptide; ATP1B1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Glu63-Ser303

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human ATP1B1 (P05026-1)(Glu63-Ser303) was expressed with an N-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ATP1B1 recombinant protein

Background: ATP1B1 belongs to the family of Na⁺/K⁺ and H⁺/K⁺-ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. ATP1B1 is a subunit of Na⁺/K⁺-ATPase. Na⁺/K⁺-ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. Na⁺/K⁺-ATPase is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). ATP1B1 regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. ATP1B1 is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with which catalyzes the hydrolysis of ATP coupled with the exchange of Na⁺ and K⁺ ions across the plasma membrane.

References

Lingrel JB. et al., 1990, Prog Nucleic Acid Res Mol Biol. 38: 37-89. Oakey RJ. et al., 1993, Hum Mol Genet. 1 (8): 613-20. Ushkaryov YuA. et al., 1990, FEBS Lett. 257 (2): 439-42.

NCBI and Uniprot Product Information

• NCBI GI #: 4502277
• NCBI GeneID: 481
• NCBI Accession #: NP_001668.1
• NCBI GenBank Nucleotide #: NM_001677.3
• UniProt Accession #: P05026; Q5TGZ3
• Molecular Weight: 34,893 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit beta-1
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, beta 1 polypeptide
• NCBI Official Symbol: ATP1B1
• NCBI Official Synonym Symbols: ATP1B
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit beta-1; Beta 1-subunit of Na(+),K(+)-ATPase; Na, K-ATPase beta-1 polypeptide; adenosinetriphosphatase; sodium pump subunit beta-1; sodium-potassium ATPase subunit beta 1 (non-catalytic); sodium/potassium-depend
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit beta-1
• UniProt Gene Name: ATP1B1
• UniProt Synonym Gene Names: ATP1B
• UniProt Entry Name: AT1B1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010]

Uniprot Description

ATP1B1: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Belongs to the X(+)/potassium ATPases subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: basolateral plasma membrane; apical plasma membrane; plasma membrane; caveola; sodium:potassium-exchanging ATPase complex; sarcolemma; vesicle

Molecular Function: protein binding; ATPase activator activity; sodium:potassium-exchanging ATPase activity; ATPase binding

Biological Process: cellular sodium ion homeostasis; protein stabilization; cellular potassium ion homeostasis; cellular calcium ion homeostasis; potassium ion import; positive regulation of ATPase activity; regulation of gene expression; response to hypoxia; blood coagulation; cell adhesion; leukocyte migration; transmembrane transport; cardiac muscle contraction

Disease: Hypertension, Essential