Typical Testing Data/Standard Curve (for reference only)
Human Glutaredoxin 5 (GLRX5) ELISA Kit | GLRX5 elisa kit
Human Glutaredoxin 5 (GLRX5) ELISA Kit
Gene Names
GLRX5; GRX5; PRSA; SIDBA3; SPAHGC; FLB4739; PR01238; PRO1238; C14orf87
Reactivity
Human
Synonyms
Glutaredoxin 5 (GLRX5); Human Glutaredoxin 5 (GLRX5) ELISA Kit; GLRX5 elisa kit
Reactivity
Human
Sequence Length
157
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
15ng/L - 3000ng/L
Sensitivity
7.08ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GLRX5 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Glutaredoxin-related Protein 5, Mitochondrial (also known as GLRX5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human GLRX5 antibody. GLRX5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human GLRX5 Antibody is added and binds to GLRX5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GLRX5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human GLRX5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human GLRX5 antibody. GLRX5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human GLRX5 Antibody is added and binds to GLRX5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GLRX5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human GLRX5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,628 Da
NCBI Official Full Name
glutaredoxin-related protein 5, mitochondrial
NCBI Official Synonym Full Names
glutaredoxin 5
NCBI Official Symbol
GLRX5
NCBI Official Synonym Symbols
GRX5; PRSA; SIDBA3; SPAHGC; FLB4739; PR01238; PRO1238; C14orf87
NCBI Protein Information
glutaredoxin-related protein 5, mitochondrial
UniProt Protein Name
Glutaredoxin-related protein 5, mitochondrial
Protein Family
UniProt Gene Name
GLRX5
UniProt Synonym Gene Names
C14orf87
NCBI Description
This gene encodes a mitochondrial protein, which is evolutionarily conserved. It is involved in the biogenesis of iron-sulfur clusters, which are required for normal iron homeostasis. Mutations in this gene are associated with autosomal recessive pyridoxine-refractory sideroblastic anemia. [provided by RefSeq, May 2010]
Uniprot Description
Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters (PubMed:20364084). Involved in protein lipoylation, acting in the pathway that provides an iron-sulfur cluster to lipoate synthase (PubMed:24334290). Required for normal iron homeostasis. Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1 (PubMed:20364084). May protect cells against apoptosis due to reactive oxygen species and oxidative stress ().
Research Articles on GLRX5
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Product Notes
The Human GLRX5 glrx5 (Catalog #AAA163278) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA163278 ELISA Kit recognizes Human GLRX5. It is sometimes possible for the material contained within the vial of "Glutaredoxin 5 (GLRX5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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