Rabbit anti-Human ATP1alpha1/Na+K+ ATPase1 Polyclonal Antibody | anti-Atp1a1 antibody

ATP1alpha1/Na+K+ ATPase1 (Ab-23) Antibody

Cat. Num. AAA9406602

• Gene Names: Atp1a1; Nkaa1b
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: ATP1alpha1/Na+K+ ATPase1; Polyclonal Antibody; ATP1alpha1/Na+K+ ATPase1 (Ab-23) Antibody; ATP1a1/Na+K+ ATPase1 (Ab-23) Antibody; A1A1; AT1A1; ATPase; Na+/K+ transporting; alpha 1 polypeptide; anti-Atp1a1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total ATP1a1/Na+K+ ATPase1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1023

• Applicable Applications for anti-Atp1a1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:3000; Immunofluorescence: 1:100~1:500
• Immunogen Type: Peptide
• Immunogen Description: Synthesized non-phosphopeptide derived from human ATP1a1/Na+K+ ATPase1 around the phosphorylation site of serine 23 (K-K-S(p)-K-K).
• Target Name: ATP1a1/Na+K+ ATPase1
• Preparation and Storage: Store at -20 degree C

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells, using ATP1a1/Na+K+ ATPase1 (Ab-23) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using ATP1a1/Na+K+ ATPase1 (Ab-23) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from K562 cells (Lane 2), using ATP1a1/Na+K+ ATPase1 (Ab-23) antiobdy. The lane on the left is treated with synthesized peptide.)

Related Product Information for anti-Atp1a1 antibody

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.Shull G.E., Biochemistry 25:8125-8132(1986).Hara Y., J. Biochem. 102:43-58(1987).Herrera V.L.M., J. Cell Biol. 105:1855-1865(1987).

Product Categories/Family for anti-Atp1a1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 6978543
• NCBI GeneID: 24211
• NCBI Accession #: NP_036636.1
• NCBI GenBank Nucleotide #: NM_012504.1
• UniProt Accession #: P06685; Q64609
• Molecular Weight: 113,054 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit alpha-1
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• NCBI Official Symbol: Atp1a1
• NCBI Official Synonym Symbols: Nkaa1b
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit alpha-1
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit alpha-1
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: Atp1a1
• UniProt Synonym Gene Names: Na(+)/K(+) ATPase alpha-1 subunit
• UniProt Entry Name: AT1A1_RAT

NCBI Description

mediates Na+ and K+ transport; may play a role in regulation of blood pressure [RGD, Feb 2006]

Uniprot Description

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.