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Western Blot (WB) (Western blot analysis of ATP12A Antibody (Center) in K562 cell line lysates (35ug/lane). ATP12A (arrow) was detected using the purified Pab.)

Rabbit anti-Human ATP12A Polyclonal Antibody | anti-ATP11A antibody

ATP12A, ID (Potassium-transporting ATPase alpha Chain 2, Proton Pump, Non-gastric H(+)/K(+) ATPase Subunit alpha, ATP1AL1)

Gene Names
ATP11A; ATPIH; ATPIS
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
ATP12A; Polyclonal Antibody; ID (Potassium-transporting ATPase alpha Chain 2; Proton Pump; Non-gastric H(+)/K(+) ATPase Subunit alpha; ATP1AL1); Anti -ATP12A; anti-ATP11A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ATP12A.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-ATP11A antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot.
Dilution: ELISA: 1:1000
Flow Cytometry: 1:10:1:50
Immunohistochemistry (paraffin): 1:50-1:100
Western Blot: 1:100-1:500
Immunogen
KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa839-868 in the center region of human ATP12A.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATP12A Antibody (Center) in K562 cell line lysates (35ug/lane). ATP12A (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of ATP12A Antibody (Center) in K562 cell line lysates (35ug/lane). ATP12A (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skin reacted with ATP12A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human skin reacted with ATP12A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ATP12A Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (ATP12A Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ATP11A antibody
ATP12A belongs to the family of P-type cation transport ATPases. This protein is a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues.
Product Categories/Family for anti-ATP11A antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
106,512 Da
NCBI Official Full Name
ATPase, Class VI, type 11A
NCBI Official Synonym Full Names
ATPase, class VI, type 11A
NCBI Official Symbol
ATP11A
NCBI Official Synonym Symbols
ATPIH; ATPIS
NCBI Protein Information
probable phospholipid-transporting ATPase IH; OTTHUMP00000018729; OTTHUMP00000062144; phospholipid-translocating ATPase; potential phospholipid-transporting ATPase IH
UniProt Protein Name
ATPase, Class VI, type 11A
UniProt Gene Name
ATP11A
UniProt Entry Name
Q5T556_HUMAN

NCBI Description

The protein encoded by this gene is an integral membrane ATPase. The encoded protein is probably phosphorylated in its intermediate state and likely drives the transport of ions such as calcium across membranes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

ATP11A: is an integral membrane ATPase. The encoded protein is probably phosphorylated in its intermediate state and likely drives the transport of ions such as calcium across membranes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Protein type: Transporter; Transporter, ion channel; EC 3.6.3.1; Membrane protein, integral; Membrane protein, multi-pass; Hydrolase

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: recycling endosome; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; lysosomal membrane; early endosome; plasma membrane; integral to membrane

Molecular Function: phospholipid-translocating ATPase activity; protein binding; magnesium ion binding; ATP binding

Biological Process: phospholipid translocation; metabolic process

Research Articles on ATP11A

Similar Products

Product Notes

The ATP11A atp11a (Catalog #AAA628974) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP12A, ID (Potassium-transporting ATPase alpha Chain 2, Proton Pump, Non-gastric H(+)/K(+) ATPase Subunit alpha, ATP1AL1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATP12A can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Dilution: ELISA: 1:1000 Flow Cytometry: 1:10:1:50 Immunohistochemistry (paraffin): 1:50-1:100 Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the ATP11A atp11a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP12A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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