Rabbit anti-Human ACE2 Polyclonal Antibody | anti-ACE2 antibody

RABBIT ANTI HUMAN ACE2 (N-TERMINAL)

Cat. Num. AAA221779

• Gene Names: ACE2; ACEH
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Synonyms: ACE2; Polyclonal Antibody; RABBIT ANTI HUMAN ACE2 (N-TERMINAL); anti-ACE2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Rabbit anti Human ACE2 antibody recognizes human Angiotensin-converting enzyme 2, also known as ACE2, ACE-related carboxypeptidase or Metalloprotease MPROT15. ACE2 is a 788 amino acid ~100 kDa carboxypeptidase expressed in a range of tissues including heart, kidney, testis and the gastrointestinal system.; ACE2 is a homologue of ACE (Angiotensin-converting enzyme 1), although it has a distinct catalytic activity. ACE2 catalyzes the cleavage of active angiotensin II into angiotensin 1-7, which may counteract the effects of ACE by increasing vasodilatation.; ACE2 serves as the cellular entry point for the severe acute respiratory syndrome (SARS) virus, functioning as a receptor for the SARS Spike protein (Turner et al. 2004).
• Form/Format: Purified IgG - liquid; Phosphate buffered saline
• Concentration: 1.0 mg/ml (varies by lot)

• Applicable Applications for anti-ACE2 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. (1)This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
• Target Species: Human
• Antiserum Preparation: Antisera to human ACE2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Preservative Stabilisers: 0.02% Sodium Azide
• Immunogen: Synthetic peptide corresponding to a sequence within the amino terminus of human ACE2.
• Preparation and Storage: Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Testing Data #2

(Immunohistochemical staining of human kidney woth Rabbit anti Human ACE2 (MBS221779))

Related Product Information for anti-ACE2 antibody

MBS221779 is specific for human Angiotensin-converting enzyme 2 (ACE2). ACE2 is a carboxypeptidase which is expressed in a range of tissues including heart, kidney, testis and the gastrointestinal system. ACE2 is a homologue of ACE (Angiotensin-converting enzyme 1), although it has a distinct catalytic activity. ACE2 catalyzes the cleavage of active angiotensin II into angiotensin 1-7, which may counteract the effects of ACE by increasing vasodilatation. A recent discovery is that ACE2 serves as the cellular entry point for the severe acute respiratory syndrome (SARS) virus, functioning as a receptor for the SARS Spike protein.

NCBI and Uniprot Product Information

• NCBI GI #: 11225609
• NCBI GeneID: 59272
• NCBI Accession #: NP_068576.1
• NCBI GenBank Nucleotide #: NM_021804.2
• UniProt Accession #: Q9BYF1; Q6UWP0; Q86WT0; Q9NRA7; Q9UFZ6; C7ECU1
• NCBI Official Full Name: angiotensin-converting enzyme 2
• NCBI Official Synonym Full Names: angiotensin I converting enzyme 2
• NCBI Official Symbol: ACE2
• NCBI Official Synonym Symbols: ACEH
• NCBI Protein Information: angiotensin-converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme homolog; metalloprotease MPROT15
• UniProt Protein Name: Angiotensin-converting enzyme 2
• Protein Family: Angiotensin-converting enzyme
• UniProt Gene Name: ACE2
• UniProt Synonym Gene Names: ACEH
• UniProt Entry Name: ACE2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

Uniprot Description

ACE2: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Interacts with ITGB1. Interacts with SARS-CoV and HCoV- NL63 spike glycoprotein. Up-regulated in failing heart. Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells. Expressed in heart, kidney, testis, and gastrointestinal system. Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Belongs to the peptidase M2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.17.23; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: extracellular space; cell surface; cytoplasm; extracellular region; integral to membrane; plasma membrane; lipid raft

Molecular Function: viral receptor activity; peptidyl-dipeptidase activity; protein binding; zinc ion binding; metallopeptidase activity; carboxypeptidase activity; peptide hormone binding; endopeptidase activity; glycoprotein binding

Biological Process: virion attachment, binding of host cell surface receptor; entry of virus into host cell; maternal process involved in pregnancy; regulation of vasodilation; angiotensin maturation; proteolysis; regulation of systemic arterial blood pressure by renin-angiotensin; regulation of cytokine production; regulation of cell proliferation; cellular protein metabolic process; regulation of vasoconstriction; regulation of inflammatory response; angiotensin catabolic process in blood; receptor biosynthetic process; angiotensin mediated drinking behavior