Rabbit anti-Human ATM Polyclonal Antibody | anti-ATM antibody

ATM Antibody (N-term)

Cat. Num. AAA9217163

• Gene Names: ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Synonyms: ATM; Polyclonal Antibody; ATM Antibody (N-term); Serine-protein kinase ATM; Ataxia telangiectasia mutated; A-T mutated; anti-ATM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This ATM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5~34 amino acids from the N-terminal region of human ATM.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Length: 3056

• Applicable Applications for anti-ATM antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: WB:~~1:500; IHC-P:~~1:50~100
• Cellular Location: Nucleus. Cytoplasmic vesicle Note=Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.
• Tissue Location: Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes
• Antigen Region: 5-34
• Preparation and Storage: Maintain refrigerated at 2-8°C. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-ATM Antibody (C12) at 1:500 dilution + Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 351 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)-Paraffin

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-ATM antibody

ATM is involved in signal transduction, cell cycle control and DNA repair, and may function as a tumor suppressor. It is necessary for activation of ABL1 and SAPK, and phosphorylates p53, NFKBIA, BRCA1, CTIP, NIBRIN (NBS1), TERF1, and RAD9. This protein has potential roles in vesicle and/or protein transport, T-cell development, gonad and neurological function. ATM is also part of the BRCA1-associated genome surveillance complex. ATM is induced by ionizing radiation. Defects in ATM are the cause of ataxia talangiectasia (AT), also known as Louis-Bar syndrome, a rare recessive disorder characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. About 30% of AT patients develop lymphomas and leukemias. Defects in ATM also contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. Defects in ATM also contribute to B-cell non-Hodgkin's lymphomas, and to B-cell chronic lymphocytic leukemia, a disease characterized by accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure.

Product Categories/Family for anti-ATM antibody

Primary Antibodies; Cancer; Neuroscience

References

Suzuki, A., et al., J. Biol. Chem. 278 (1):48-53 (2003). Kishi, S., et al., J. Biol. Chem. 276 (31):29282-29291 (2001). Schaffner, C., et al., Proc. Natl. Acad. Sci. U.S.A. 97 (6):2773-2778 (2000). Gatei, M., et al., Nat. Genet. 25 (1):115-119 (2000). Becker-Catania, S.G., et al., Mol. Genet. Metab. 70 (2):122-133 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 71902540
• NCBI GeneID: 472
• NCBI Accession #: NP_000042.3
• NCBI GenBank Nucleotide #: NM_000051.3
• UniProt Accession #: Q13315; O15429; Q12758; Q16551; Q93007; Q9NP02; Q9UCX7; B2RNX5
• NCBI Official Full Name: serine-protein kinase ATM
• NCBI Official Synonym Full Names: ATM serine/threonine kinase
• NCBI Official Symbol: ATM
• NCBI Official Synonym Symbols: AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
• NCBI Protein Information: serine-protein kinase ATM
• UniProt Protein Name: Serine-protein kinase ATM
• Protein Family: ATM interactor
• UniProt Gene Name: ATM
• UniProt Synonym Gene Names: A-T mutated
• UniProt Entry Name: ATM_HUMAN

NCBI Description

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Uniprot Description

ATM: an atypical kinase of the PIKK family. Regulates cell cycle checkpoints and DNA repair . May function as a tumor suppressor. Activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Involved in the activation of ABL1 and SAPK. Binds DNA ends and is part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. DNA damage promotes association with RAD17. LOF mutations associated with ataxia telangiectasia, causing progressive loss of motor control (ataxia), dilation of superficial blood vessels (telangiectasia), cancer and immune deficiency. Approximately 30% of cases develop tumors, mostly lymphomas and leukemias, due to defects in DNA damage repair. Somatic mutations seen in leukemias and lymphomas.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; Tumor suppressor; DNA repair, damage; EC 2.7.11.1; ATYPICAL group; PIKK family

Chromosomal Location of Human Ortholog: 11q22-q23

Cellular Component: chromosome, telomeric region; cytoplasmic membrane-bound vesicle; nuclear chromosome, telomeric region; nucleoplasm; nucleus; spindle

Molecular Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; DNA binding; DNA-dependent protein kinase activity; protein binding; protein complex binding; protein dimerization activity; protein N-terminus binding; protein serine/threonine kinase activity

Biological Process: brain development; cell cycle arrest; determination of adult life span; DNA damage induced protein phosphorylation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction resulting in induction of apoptosis; DNA double-strand break processing; DNA repair; DNA replication; DNA synthesis during DNA repair; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; heart development; histone phosphorylation; lipoprotein catabolic process; meiotic recombination; mitotic cell cycle spindle assembly checkpoint; negative regulation of B cell proliferation; neuron apoptosis; oocyte development; peptidyl-serine phosphorylation; positive regulation of apoptosis; positive regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of histone phosphorylation; positive regulation of neuron apoptosis; positive regulation of telomere maintenance via telomerase; pre-B cell allelic exclusion; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of autophagy; regulation of telomerase activity; response to DNA damage stimulus; response to hypoxia; response to ionizing radiation; signal transduction; somitogenesis; strand displacement; telomere clustering; telomere maintenance via telomerase; V(D)J recombination

Disease: Ataxia-telangiectasia; Breast Cancer