Immunoprecipitation (IP)
(ATL3 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-ATL3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ATL3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 61 kDaObserved band size: 61 kDa)
Rabbit anti-Human ATL3 Polyclonal Antibody | anti-ATL3 antibody
Anti-ATL3 Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate
Immunoprecipitation (IP)
(ATL3 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-ATL3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ATL3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 61 kDaObserved band size: 61 kDa)
Immunoprecipitation (IP)
(ATL3 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-ATL3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ATL3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 61 kDaObserved band size: 61 kDa)
Western Blot (WB)
(Anti-ATL3 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse liver Tissue LysateLane B: 293T Whole Cell LysateLane C: NIH-3T3 Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:61 kDaObserved band size:61 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-ATL3 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse liver Tissue LysateLane B: 293T Whole Cell LysateLane C: NIH-3T3 Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:61 kDaObserved band size:61 kDa(We are unsure as to the identity of these extra bands.))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a family of dynamin-like, integral membrane GTPases. The encoded protein is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. Mutations in this gene may be associated with hereditary sensory neuropathy type IF. Alternatively spliced transcript variants that encode distinct isoforms have been described. [provided by RefSeq, Feb 2014]
Uniprot Description
ATL3: GTPase tethering membranes through formation of trans- homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. Belongs to the GBP family. Atlastin subfamily.
Protein type: Membrane protein, multi-pass; EC 3.6.5.-; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane
Molecular Function: GTPase activity; identical protein binding; GTP binding
Biological Process: endoplasmic reticulum organization and biogenesis; ER to Golgi vesicle-mediated transport; metabolic process; Golgi organization and biogenesis; protein homooligomerization
Disease: Neuropathy, Hereditary Sensory, Type If
Research Articles on ATL3
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Product Notes
The ATL3 atl3 (Catalog #AAA8107597) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ATL3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATL3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the ATL3 atl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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