Human Interferon Gamma Inducible Protein 30 (IFI30) ELISA Kit | IFI30 elisa kit

Human Interferon Gamma Inducible Protein 30 (IFI30) ELISA Kit

Cat. Num. AAA452623

• Gene Names: IFI30; GILT; IP30; IP-30; IFI-30
• Reactivity: Human
• Synonyms: Interferon Gamma Inducible Protein 30 (IFI30); Human Interferon Gamma Inducible Protein 30 (IFI30) ELISA Kit; GILT; IP30; Legumaturain; Gamma-Interferon-Inducible Lysosomal Thiol Reductase; Gamma-interferon-inducible lysosomal thiol reductase; IFI30 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IFI30. No significant cross-reactivity or interference between IFI30 and analogues was observed.
• Sequence Length: 250

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.064ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IFI30 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IFI30 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IFI30 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IFI30 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFI30. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IFI30. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IFI30, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IFI30 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 29150261
• NCBI GeneID: 10437
• NCBI Accession #: NP_006323.2
• NCBI GenBank Nucleotide #: NM_006332.4
• UniProt Accession #: P13284; Q76MF9; Q8NEI4; Q8WU77; Q9UL08
• Molecular Weight: 27,964 Da
• NCBI Official Full Name: gamma-interferon-inducible lysosomal thiol reductase preproprotein
• NCBI Official Synonym Full Names: IFI30, lysosomal thiol reductase
• NCBI Official Symbol: IFI30
• NCBI Official Synonym Symbols: GILT; IP30; IP-30; IFI-30
• NCBI Protein Information: gamma-interferon-inducible lysosomal thiol reductase
• UniProt Protein Name: Gamma-interferon-inducible lysosomal thiol reductase
• Protein Family: Gamma-interferon-inducible lysosomal thiol reductase
• UniProt Gene Name: IFI30
• UniProt Synonym Gene Names: GILT; IP30

NCBI Description

The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. [provided by RefSeq, Jul 2008]

Uniprot Description

Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds (). Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation ().