Sheep ATIII - Antithrombin Polyclonal Antibody | anti-Serpinc1 antibody

Sheep anti-human Antithrombin (ATIII), Whole IgG from antiserum

Cat. Num. AAA512015

• Gene Names: Serpinc1; At3; At-3; ATIII; AI114908
• Purity: Purified IgG.; Vial containing ml of whole IgG representing approximately 1 ml of antiserum. Total protein is 10 mg.
• Synonyms: ATIII - Antithrombin; Polyclonal Antibody; Sheep anti-human Antithrombin (ATIII); Whole IgG from antiserum; human; anti-Serpinc1 antibody

• Host: Sheep
• Clonality: Polyclonal
• Specificity: This antibody is specific for antithrombin as demonstrated by immunoelectrophoresis and ELISA.
• Purity/Purification: Purified IgG.; Vial containing ml of whole IgG representing approximately 1 ml of antiserum. Total protein is 10 mg.
• Form/Format: Whole IgG, clear liquid.
• Concentration: IgG concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 13.4. (varies by lot)

• Application Notes: Suitable as a source of antibodies to human antithrombin.
• Immunogen: Human antithrombin purified from plasma.
• Buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol.
• Neutralizing Activity: Not determined
• Preparation and Storage: Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.

Related Product Information for anti-Serpinc1 antibody

Antithrombin, also known as Antithrombin III (ATIII), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of thrombin in plasma. It is produced in the liver and circulates in plasma at ~200 ug/ml (~3.5 uM). Antithrombin inhibits a broad spectrum of serine proteases including thrombin, activated forms of factor X, factor IX, factor XI, factor XII, as well as kallikrein, plasmin and urokinase. Enzyme inhibition by antithrombin occurs through proteolytic cleavage at Arg385-Ser386 and subsequent rapid formation of a stable, inactive 1:1 enzyme-antithrombin complex. Heparin has a profound accelerating effect on the inhibitory activity of antithrombin towards some enzymes. For example, the rate of inhibition of thrombin and activated factor X is increased 1000-fold in the presence of optimal concentrations of heparin, whereas heparin has relatively little effect on the inhibition rate of activated factor XI, activated factor XII and kallikrein. Antithrombin is a single chain molecule with a molecular weight of 59 kDa. Interaction with thrombin results in an SDS-stable thrombin-antithrombin complex of 96 kDa1-3.

References

1. Damus PS, Rosenberg RD; Antithrombin - Heparin Cofactor; Methods in Enzymology 45, pp 653-669, 1976.
2. Harpel PC; Blood Proteolytic Enzyme Inhibitors: Their Role in Modulating Blood Coagulation and Fibrinolytic Enzyme Pathways; in Hemostasis and Thrombosis, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 738- 747, J.B. Lippincott Co., Philadelphia PA, USA, 1982.
3. Griffith MJ, Noyes CM, Church FC; Reactive Site Peptide Structural Similarity between Heparin Cofactor II and Antithrombin III; JBC:260, pp 2218-2225, 1985.

NCBI and Uniprot Product Information

• NCBI GI #: 258959
• NCBI GeneID: 11905
• UniProt Accession #: P32261; Q543J5
• Molecular Weight: 52,004 Da
• NCBI Official Full Name: antithrombin III
• NCBI Official Synonym Full Names: serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1
• NCBI Official Symbol: Serpinc1
• NCBI Official Synonym Symbols: At3; At-3; ATIII; AI114908
• NCBI Protein Information: antithrombin-III; serpin C1; anti-thrombin 3; serpin peptidase inhibitor, clade C, member 1; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
• UniProt Protein Name: Antithrombin-III
• Protein Family: Antithrombin
• UniProt Gene Name: Serpinc1
• UniProt Synonym Gene Names: At3
• UniProt Entry Name: ANT3_MOUSE

Uniprot Description

Function: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.

Subcellular location: Secreted › extracellular space.

Tissue specificity: Plasma.

Post-translational modification: Phosphorylation sites are present in the extracelllular medium

Sequence similarities: Belongs to the serpin family.