Chemiluminescence
(Staining of Human Adipose lysate (35ug protein in RIPA buffer) using MBS644527 (0.5ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat ATGL Polyclonal Antibody
ATGL (Patatin-like Phospholipase Domain-containing Protein 2, Adipose Triglyceride Lipase, Calcium-independent Phospholipase A2, Desnutrin, IPLA2-zeta, Pigment Epithelium-derived Factor, TTS2.2, Transport-secretion Protein 2, TTS2, PNPLA2, ATGL, FP17548)
Reactivity
Bovine, Canine, Human, Mouse, Porcine, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
ATGL; Polyclonal Antibody; ATGL (Patatin-like Phospholipase Domain-containing Protein 2; Adipose Triglyceride Lipase; Calcium-independent Phospholipase A2; Desnutrin; IPLA2-zeta; Pigment Epithelium-derived Factor; TTS2.2; Transport-secretion Protein 2; TTS2; PNPLA2; FP17548); Anti -ATGL (Patatin-like Phospholipase Domain-containing Protein 2; anti-ATGL antibody
Host
Goat
Reactivity
Bovine, Canine, Human, Mouse, Porcine, Rat
Clonality
Polyclonal
Specificity
Recognizes human PNPLA2. Species sequence homology: Bovine, canine, mouse, porcine and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Applicable Applications for anti-ATGL antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 1:32,000
Western Blot: 0.5-2ug/ml, observed in human adipose, heart and testis lysates on ~60kD bands
Immunohistochemistry (Paraffin): 2-3ug/ml
Dilution: ELISA: 1:32,000
Western Blot: 0.5-2ug/ml, observed in human adipose, heart and testis lysates on ~60kD bands
Immunohistochemistry (Paraffin): 2-3ug/ml
Immunogen
Synthetic peptide corresponding to C-NIIEVSKEARKR, from human PNPLA2, at the internal region of the protein (NP_065109.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chemiluminescence
(Staining of Human Adipose lysate (35ug protein in RIPA buffer) using MBS644527 (0.5ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.)
Chemiluminescence
(Staining of Human Adipose lysate (35ug protein in RIPA buffer) using MBS644527 (0.5ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
(Staining of paraffin embedded Human Heart using MBS644527 (2.5ug/ml). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(Staining of paraffin embedded Human Heart using MBS644527 (2.5ug/ml). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Related Product Information for anti-ATGL antibody
Triglycerides are the most efficient form of energy storage in mammalian adipose tissue during times of caloric excess. ATGL is one of the key enzymes involved in the mobilization of fatty acids from triglyceride stores in adipose tissue, catalyzing the conversion of triacylglycerols to diacylglycerols. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity, and thus may be a potential drug target for the diabetic pathology. ATGL mRNA is detected in a wide range of tissues including adipose, lung, skeletal muscle, testis, heart, brain, and kidney, with adipose tissue expressing the highest level.
Product Categories/Family for anti-ATGL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
ATGL
Similar Products
Product Notes
The ATGL (Catalog #AAA644527) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The ATGL (Patatin-like Phospholipase Domain-containing Protein 2, Adipose Triglyceride Lipase, Calcium-independent Phospholipase A2, Desnutrin, IPLA2-zeta, Pigment Epithelium-derived Factor, TTS2.2, Transport-secretion Protein 2, TTS2, PNPLA2, ATGL, FP17548) reacts with Bovine, Canine, Human, Mouse, Porcine, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATGL can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: ELISA: 1:32,000 Western Blot: 0.5-2ug/ml, observed in human adipose, heart and testis lysates on ~60kD bands Immunohistochemistry (Paraffin): 2-3ug/ml. Researchers should empirically determine the suitability of the ATGL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATGL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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