Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of WDR5 in 293 cell lysate with WDR5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

Rabbit anti-Human WDR5 Polyclonal Antibody | anti-WDR5 antibody

WDR5 Antibody

Gene Names
WDR5; SWD3; BIG-3; CFAP89
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
WDR5 Antibody is affinity chromatography purified via peptide column.
Synonyms
WDR5; Polyclonal Antibody; WDR5 Antibody; SWD3; BIG-3; BIG3; WD repeat-containing protein 5; BMP2-induced 3-kb gene protein; WD repeat domain 5; anti-WDR5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
WDR5 antibody is human specific. WDR5 antibody is predicted to not cross-react with other WDR family members.
Purity/Purification
WDR5 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
334
Applicable Applications for anti-WDR5 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WDR5 antibody can be used for detection of WDR5 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 5 mug/mL.
Conjugate
Unconjugated
Immunogen
WDR5 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human WDR5.
Buffer
WDR5 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
WDR5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of WDR5 in 293 cell lysate with WDR5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

Western Blot (WB) (Western blot analysis of WDR5 in 293 cell lysate with WDR5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

Immunocytochemistry (IHC)

(Immunocytochemistry of WDR5 in 293 cells with WDR5 antibody at 5 μg/mL.)

Immunocytochemistry (IHC) (Immunocytochemistry of WDR5 in 293 cells with WDR5 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of WDR5 in 293 cells with WDR5 antibody at 20 μg/mL.)

Immunofluorescence (IF) (Immunofluorescence of WDR5 in 293 cells with WDR5 antibody at 20 μg/mL.)
Related Product Information for anti-WDR5 antibody
WDR5 Antibody: WD repeat domain 5 (WDR5) is a member of the WD repeat protein family, which is involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WDR5, also known as BIG-3, is expressed in the developing growth plate, accelerates chondrocyte and osteoblast differentiation in vitro, and regulates osteoblast differentiation during embryonic bone development. WDR5 interacts with the pluripotency factor Oct4/POU5F1 and is required for the efficient formation of induced pluripotent stem (iPS) cells.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,588 Da
NCBI Official Full Name
WD repeat-containing protein 5
NCBI Official Synonym Full Names
WD repeat domain 5
NCBI Official Symbol
WDR5
NCBI Official Synonym Symbols
SWD3; BIG-3; CFAP89
NCBI Protein Information
WD repeat-containing protein 5; WD-repeat protein 5; BMP2-induced 3-kb gene protein; SWD3, Set1c WD40 repeat protein, homolog; cilia and flagella associated protein 89
UniProt Protein Name
WD repeat-containing protein 5
UniProt Gene Name
WDR5
UniProt Synonym Gene Names
BIG3
UniProt Entry Name
WDR5_HUMAN

NCBI Description

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 7 WD repeats. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

WDR5: a WD40 repeat protein. May accelerate osteoblast differentiation. Interacts with HCFC1. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: nucleoplasm; histone methyltransferase complex; nucleus; histone acetyltransferase complex

Molecular Function: protein binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone acetyltransferase activity (H4-K16 specific)

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; histone H3-K4 methylation; skeletal development

Research Articles on WDR5

Similar Products

Product Notes

The WDR5 wdr5 (Catalog #AAA151253) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WDR5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WDR5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WDR5 antibody can be used for detection of WDR5 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 5 mug/mL. Researchers should empirically determine the suitability of the WDR5 wdr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WDR5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.