Rabbit ATG9A Polyclonal Antibody | anti-ATG9A antibody

ATG9A Antibody

Cat. Num. AAA9217096

• Gene Names: ATG9A; mATG9; APG9L1; MGD3208
• Reactivity: Human; Predicted Reactivity: Bovine, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Synonyms: ATG9A; Polyclonal Antibody; ATG9A Antibody; Autophagy-related protein 9A; APG9-like 1; mATG9; APG9L1; anti-ATG9A antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Bovine, Rat
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This ATG9A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 723~753 amino acids surrounding S738 of human APG9L1.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 839

• Applicable Applications for anti-ATG9A antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: WB:~~1:1000; IHC-P:~~1:10~50
• Subtitle: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation- induced redistribution depends on ULK1, ATG13, as well as SH3GLB1.
• Antigen Region: 723-753 aa
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-APG9L1 Antibody (S738) at 1:1000 dilutionLane 1: MCF-7 whole cell lysateLane 2: HepG2 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 94kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)-Paraffin

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with APG9L1 Antibody (S738), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-ATG9A antibody

Description: Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

Background: Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.

Product Categories/Family for anti-ATG9A antibody

Primary Antibodies; Cancer; Cardiovascular; Cell Biology; Metabolism; Neuroscience; Signal Transduction

References

Baehrecke EH. Nat Rev Mol Cell Biol. 6 (6):505-10. (2005). Lum JJ, et al. Nat Rev Mol Cell Biol. 6 (6):439-48. (2005). Greenberg JT. Dev Cell. 8 (6):799-801. (2005). Levine B. Cell. 120 (2):159-62. (2005). Shintani T and Klionsky DJ. Science. 306 (5698):990-5. (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 116089282
• NCBI GeneID: 79065
• NCBI Accession #: NP_001070666.1
• NCBI GenBank Nucleotide #: NM_001077198.2
• UniProt Accession #: Q7Z3C6; Q3ZAQ6; Q6P0N7; Q7Z317; Q7Z320; Q8NDK6; Q8WU65; Q9BVL5; Q9H6L1; Q9HAG7
• Molecular Weight: 94447 Da
• NCBI Official Full Name: autophagy-related protein 9A
• NCBI Official Synonym Full Names: autophagy related 9A
• NCBI Official Symbol: ATG9A
• NCBI Official Synonym Symbols: mATG9; APG9L1; MGD3208
• NCBI Protein Information: autophagy-related protein 9A
• UniProt Protein Name: Autophagy-related protein 9A
• Protein Family: Autophagy-related protein
• UniProt Gene Name: ATG9A
• UniProt Synonym Gene Names: APG9L1
• UniProt Entry Name: ATG9A_HUMAN

Uniprot Description

ATG9A: Plays a role in autophagy. Cycles between a juxta- nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and FAM48A. Interacts with FAM48A. Belongs to the ATG9 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Autophagy; Vesicle

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: cytoplasmic vesicle; endoplasmic reticulum membrane; endosome; integral to membrane; late endosome; late endosome membrane; membrane; recycling endosome; trans-Golgi network

Molecular Function: protein binding

Biological Process: autophagic vacuole formation; mitochondrion degradation; protein transport