Rabbit ATG7 Polyclonal Antibody | anti-ATG7 antibody

ATG7 Antibody

Cat. Num. AAA809436

• Gene Names: ATG7; GSA7; APG7L; APG7-LIKE
• Reactivity: Human; Mouse; Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Peptide Affinity Purified
• Synonyms: ATG7; Polyclonal Antibody; ATG7 Antibody; Rabbit Anti-Human ATG7 Polyclonal; Autophagy 7S. cerevisiae; homolog of Antibody; APG7 like Antibody; ATG7_HUMAN Antibody; hAGP7 Antibody; Ubiquitin-like modifier-activating enzyme ATG7 Antibody; Autophagy related protein 7 Antibody; Autophagy-related protein 7 Antibody; GSA 7 Antibody; APG7; S. cerevisiae; Ubiquitin-activating enzyme E1-like protein Antibody; Autophagy-related 7 (yeast) Antibody; APG7 autophagy 7 like Antibody; 1810013K23Rik Antibody; APG7 autophagy 7-like (S. cerevisiae) Antibody; ATG12-activating enzyme E1 ATG7 Antibody; ATG7 autophagy related 7 homolog Antibody; Atg7l Antibody; Ubiquitin activating enzyme E1 like protein Antibody; Apg 7 Antibody; APG7L Antibody; DKFZp434N0735 Antibody; ATG 7 Antibody; APG7-like Antibody; GSA7 Antibody; ATG7 autophagy related 7 homolog (S. cerevisiae) Antibody; anti-ATG7 antibody

• Host: Rabbit
• Reactivity: Human; Mouse; Rat
• Clonality: Polyclonal
• Specificity: Detects 77.9 kDa.
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 676

• Applicable Applications for anti-ATG7 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000; ICC/IF: 1:100
• Target: ATG7
• Immunogen: Synthetic peptide from the N-terminal of Human ATG7
• Immunogen Species: Human
• Cellular Localization: Cytoplasm; Preautophagosomal Structure
• Conjugate: Unconjugated
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Tissue Specificity: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
• Preparation and Storage: Store at -20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG7 Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure, Organelle membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG7 Antibody. (D) Composite.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-ATG10 Polyclonal Antibody . Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.)

Western Blot (WB)

(Western blot analysis of Rat brain cell lysates showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain cell lysates. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa.)

Western Blot (WB)

(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Cervical cancer cell line (HeLa) lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa.)

Related Product Information for anti-ATG7 antibody

ATG7 in conjuction with ATG10, mediates the formation of the autophagosome when ATG12 is covalently bound to ATG5 and targets to autophagosome vesicles. It also activates ATG8, and is crucial for amino acid supply in neonates.

Product Categories/Family for anti-ATG7 antibody

Antibodies; Cancer; Apoptosis; Autophagy; Cardiovascular System; Heart

References

1. Mizushima N., et al. (1998) J Biol Chem. 273: 33889-92. 2. Mizushima N., et al. (1998) Nature. 395: 395-8. 3. Suzuki K., et al. (2001) EMBO J. 20: 5971-81. 4. Tanida I., et al. (1999) Mol Biol Cell. 10: 1367-79. 5. Shintani T., et al. (1999) EMBO J. 18: 5234-41.

NCBI and Uniprot Product Information

• NCBI GI #: 222144227
• NCBI GeneID: 10533
• NCBI Accession #: NP_001129503.2
• NCBI GenBank Nucleotide #: NM_001136031.2
• UniProt Accession #: O95352; Q7L8L0; Q9BWP2; Q9UFH4; B4E170; E9PB95
• Molecular Weight: 68,618 Da
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme ATG7 isoform b
• NCBI Official Synonym Full Names: autophagy related 7
• NCBI Official Symbol: ATG7
• NCBI Official Synonym Symbols: GSA7; APG7L; APG7-LIKE
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme ATG7
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme ATG7
• Protein Family: Ubiquitin-like modifier-activating enzyme
• UniProt Gene Name: ATG7
• UniProt Synonym Gene Names: APG7L; APG7-like; hAGP7

NCBI Description

This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.