Rabbit anti-Human ADCY10 Polyclonal Antibody | anti-ADCY10 antibody

ADCY10, ID (ADCY10, SAC, Adenylate cyclase type 10, AH-related protein, Adenylate cyclase homolog, Germ cell soluble adenylyl cyclase, Testicular soluble adenylyl cyclase) (Azide free) (HRP)

Cat. Num. AAA6271677

• Gene Names: ADCY10; SAC; HCA2; SACI; Sacy; hsAC; HEL-S-7a
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ADCY10; Polyclonal Antibody; ID (ADCY10; SAC; Adenylate cyclase type 10; AH-related protein; Adenylate cyclase homolog; Germ cell soluble adenylyl cyclase; Testicular soluble adenylyl cyclase) (Azide free) (HRP); Testicular soluble adenylyl cyclase; anti-ADCY10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ADCY10 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ADCY10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-591 amino acids from the Central region of human ADCY10.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ADCY10 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ADCY10 antibody detected the ADCY10 protein (arrow).)

Flow Cytometry (FC/FACS)

(ADCY10 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ADCY10 antibody

Antibodies; Enzymes; Cyclase

NCBI and Uniprot Product Information

• NCBI GI #: 268370055
• NCBI GeneID: 55811
• NCBI Accession #: NP_001161221.1; NP_060887.2
• NCBI GenBank Nucleotide #: NM_001167749.2
• UniProt Accession #: Q96PN6; O95558; Q5R329; Q5R330; Q8WXV4; Q9NNX0; B4DZF0; F5GWS5
• Molecular Weight: 169,750 Da
• NCBI Official Full Name: adenylate cyclase type 10 isoform 2
• NCBI Official Synonym Full Names: adenylate cyclase 10
• NCBI Official Symbol: ADCY10
• NCBI Official Synonym Symbols: SAC; HCA2; SACI; Sacy; hsAC; HEL-S-7a
• NCBI Protein Information: adenylate cyclase type 10
• UniProt Protein Name: Adenylate cyclase type 10
• Protein Family: Adenylate cyclase
• UniProt Gene Name: ADCY10
• UniProt Synonym Gene Names: SAC; hsAC; sAC
• UniProt Entry Name: ADCYA_HUMAN

NCBI Description

The protein encoded by this gene belongs to a distinct class of adenylyl cyclases that is soluble and insensitive to G protein or forskolin regulation. Activity of this protein is regulated by bicarbonate. Variation at this gene has been observed in patients with absorptive hypercalciuria. Alternatively spliced transcript variants encoding different isoforms have been observed. There is a pseudogene of this gene on chromosome 6. [provided by RefSeq, Jul 2014]

Uniprot Description

SAC: Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor. Involved in ciliary beat regulation. Genetic variations in ADCY10 are a cause of susceptibility to hypercalciuria absorptive type 2 (HCA2). Absorptive hypercalciuria is a common type of hypercalciuria, a condition characterized by excessive urinary calcium excretion. Absorptive hypercalciuria is due to gastrointestinal hyperabsorption of calcium and is a frequent cause of calcium oxalate nephrolithiasis. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adenylyl cyclase; Nucleotide Metabolism - purine; Lyase; EC 4.6.1.1

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: growth cone; cytoskeleton; cell soma; perinuclear region of cytoplasm; apical part of cell; axon; dendrite; cytoplasm; plasma membrane; cytosol

Molecular Function: manganese ion binding; magnesium ion binding; ATPase binding; ATP binding; adenylate cyclase activity

Biological Process: positive regulation of apoptosis; cAMP biosynthetic process; spermatogenesis

Disease: Hypercalciuria, Absorptive, 2