Rabbit anti-Mouse, Rat ATG3 Polyclonal Antibody | anti-ATG3 antibody
RABBIT ANTI ATG3
Gene Names
Atg3; APG3; Apg3l; Atg3l; PC3-96; 2610016C12Rik
Reactivity
Mouse, Rat
Applications
Immunoprecipitation, Western Blot
Synonyms
ATG3; Polyclonal Antibody; RABBIT ANTI ATG3; anti-ATG3 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This item specifically recognises ATG3 (Autophagy-related protein 3), otherwise known as APG3, a widely expressed E2-like enzyme, and member of the ATG3 family, which plays a key role in autophagosome formation, by catalyzing the conjugation of ATG8-like proteins to phosphatidylethanolamine (PE), in conjunction with ATG7.
ATG3 also plays a role in the regulation of mitochondrial homeostasis and cell death, through conjugation with ATG12 at Lys243.
This item has been shown to be suitable for use in immunoprecipitation using rat NRK cell lysates.
ATG3 also plays a role in the regulation of mitochondrial homeostasis and cell death, through conjugation with ATG12 at Lys243.
This item has been shown to be suitable for use in immunoprecipitation using rat NRK cell lysates.
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
314
Applicable Applications for anti-ATG3 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Western Blot: This item detects a band of approximately 36kDa in HeLa and mouse 3T3 cell lysates.
Immunoprecipitation: Minimum Dilution: 5; Maximum Dilution: 10ul
Western Blotting: Minimum Dilution: 2.0; Maximum Dilution: 4.0ug/ml
Immunoprecipitation: Minimum Dilution: 5; Maximum Dilution: 10ul
Western Blotting: Minimum Dilution: 2.0; Maximum Dilution: 4.0ug/ml
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide corresponding to amino acids 17-32 of human ATG3. The corresponding sequence is identical in rat and mouse.
Buffer Solution
Buffer Solution
Antiserum Preparation
Antiserum to human ATG3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-ATG3 antibody
MBS224303 specifically recognises ATG3 (Autophagy-related protein 3), otherwise known as APG3, a widely expressed E2-like enzyme, and member of the ATG3 family, which plays a key role in autophagosome formation, by catalyzing the conjugation of ATG8-like proteins to phosphatidylethanolamine (PE), in conjunction with ATG7. ATG3 also plays a role in the regulation of mitochondrial homeostasis and cell death, through conjugation with ATG12 at Lys243. MBS224303 has been shown to be suitable for use in immunoprecipitation using rat NRK cell lysates.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,796 Da
NCBI Official Full Name
ubiquitin-like-conjugating enzyme ATG3
NCBI Official Synonym Full Names
autophagy related 3
NCBI Official Symbol
Atg3
NCBI Official Synonym Symbols
APG3; Apg3l; Atg3l; PC3-96; 2610016C12Rik
NCBI Protein Information
ubiquitin-like-conjugating enzyme ATG3; autophagy-related 3; autophagy-related protein 3
UniProt Protein Name
Ubiquitin-like-conjugating enzyme ATG3
Protein Family
UniProt Gene Name
Atg3
UniProt Synonym Gene Names
Apg3l; APG3-like
UniProt Entry Name
ATG3_MOUSE
Uniprot Description
ATG3: an E2-like enzyme essential for Apg8p lipidation.
Protein type: Ubiquitin conjugating system; EC 6.3.2.-; Autophagy; Enzyme, cellular metabolism
Cellular Component: cytoplasmic ubiquitin ligase complex; cytoplasm; cytosol
Molecular Function: protein binding; enzyme binding; small conjugating protein ligase activity; coenzyme F420-2 alpha-glutamyl ligase activity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; APG8 conjugating enzyme activity; ribosomal S6-glutamic acid ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; APG12 conjugating enzyme activity; ligase activity
Biological Process: protein transport; transport; mitochondrion degradation; autophagy; protein ubiquitination; protein modification process; mitochondrial fragmentation during apoptosis; autophagic vacuole formation
Research Articles on ATG3
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Product Notes
The ATG3 atg3 (Catalog #AAA224303) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI ATG3 reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATG3 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Western Blot: This item detects a band of approximately 36kDa in HeLa and mouse 3T3 cell lysates. Immunoprecipitation: Minimum Dilution: 5; Maximum Dilution: 10ul Western Blotting: Minimum Dilution: 2.0; Maximum Dilution: 4.0ug/ml. Researchers should empirically determine the suitability of the ATG3 atg3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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