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Testing Data (Peptide-+)

Rabphilin 3A Antibody | anti-RP3A antibody

Rabphilin 3A (Ab-Ser237) Antibody

Gene Names
Rph3a; AU022689; AW108370; 2900002P20Rik
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Rabphilin 3A; Rabphilin 3A (Ab-Ser237) Antibody; RPH3A; exophilin 1; anti-RP3A antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
Rabphilin 3A (Ab-237) antibody detects endogenous levels of total rabphilin 3A protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
681
Applicable Applications for anti-RP3A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human rabphilin 3A around the phosphorylation site of serine 234 (R-A-SP-E-A).
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(Peptide-+)

Testing Data (Peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue using Rabphilin 3A (Ab-237) antibody. Western blot analysis of extracts from HeLa cells, treated with TNF-a (20ng/ml, 2mins), using Rabphilin 3A (Ab-237) antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using Rabphilin 3A (Ab-237) antibody. Western blot analysis of extracts from HeLa cells, treated with TNF-a (20ng/ml, 2mins), using Rabphilin 3A (Ab-237) antibody.)

Immunofluorescence (IF)

(TNF-a +- Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using Rabphilin 3A (Ab-237) antibody.)

Immunofluorescence (IF) (TNF-a +- Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using Rabphilin 3A (Ab-237) antibody.)
References
H Shirataki, Mol. Cell. Biol., Apr 1993; 13: 2061-2068. N Masumoto, J. Cell Biol., Dec 1996; 135: 1741. M.E. Burns, J. Gen. Physiol., Feb 1998; 111: 243.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
75,489 Da
NCBI Official Full Name
rabphilin-3A
NCBI Official Synonym Full Names
rabphilin 3A
NCBI Official Symbol
Rph3a
NCBI Official Synonym Symbols
AU022689; AW108370; 2900002P20Rik
NCBI Protein Information
rabphilin-3A
UniProt Protein Name
Rabphilin-3A
UniProt Gene Name
Rph3a

Uniprot Description

Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.

Research Articles on RP3A

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Product Notes

The RP3A rph3a (Catalog #AAA859683) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Rabphilin 3A (Ab-Ser237) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Rabphilin 3A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000. Researchers should empirically determine the suitability of the RP3A rph3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rabphilin 3A, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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