Rabbit anti-Human ATG16L Polyclonal Antibody | anti-ATG16L antibody

ATG16L, CT (ATG16L1, APG16L, Autophagy-related protein 16-1, APG16-like 1) (MaxLight 650)

Cat. Num. AAA6276037

• Gene Names: ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: ATG16L; Polyclonal Antibody; CT (ATG16L1; APG16L; Autophagy-related protein 16-1; APG16-like 1) (MaxLight 650); ATG16L1; APG16-like 1; anti-ATG16L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-ATG16L antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377~407 amino acids from the C-term of human APG16L.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hAPG16L-K366 in NIH-3T3, HepG2, Hela, Jurkat and NCI-H460 cell line lysates (35ug/lane). APG16L (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-ATG16L antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is esential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane.

Product Categories/Family for anti-ATG16L antibody

Antibodies; Autophagy

References

Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005). Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005). Greenberg JT. Dev Cell. 8(6):799-801. (2005). Levine B. Cell. 120(2):159-62. (2005). Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 298229005
• NCBI GeneID: 55054
• NCBI Accession #: NP_001177195.1
• NCBI GenBank Nucleotide #: NM_001190266.1
• UniProt Accession #: Q676U5; Q6IPN1; Q6UXW4; Q6ZVZ5; Q8NCY2; Q96JV5; Q9H619; A3EXK9; A3EXL0; B6ZDH0
• Molecular Weight: 68,265 Da
• NCBI Official Full Name: autophagy-related protein 16-1 isoform 4
• NCBI Official Synonym Full Names: autophagy related 16-like 1 (S. cerevisiae)
• NCBI Official Symbol: ATG16L1
• NCBI Official Synonym Symbols: IBD10; WDR30; APG16L; ATG16A; ATG16L
• NCBI Protein Information: autophagy-related protein 16-1; APG16L beta; WD repeat domain 30; ATG16 autophagy related 16-like 1
• UniProt Protein Name: Autophagy-related protein 16-1
• UniProt Gene Name: ATG16L1
• UniProt Synonym Gene Names: APG16L
• UniProt Entry Name: A16L1_HUMAN

NCBI Description

The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010]

Uniprot Description

ATG16: Plays an essential role in autophagy. Homooligomer. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Belongs to the WD repeat ATG16 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, peripheral; Adaptor/scaffold; Autophagy

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: pre-autophagosomal structure membrane; autophagic vacuole; axoneme

Molecular Function: identical protein binding; protein binding

Biological Process: protein transport; protein homooligomerization; autophagic vacuole formation

Disease: Inflammatory Bowel Disease 10