Rabbit anti-Human ATG16L Polyclonal Antibody | anti-ATG16L1 antibody

ATG16L, CT (ATG16L1, APG16L, Autophagy-related protein 16-1, APG16-like 1)

Cat. Num. AAA649258

• Gene Names: ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: ATG16L; Polyclonal Antibody; CT (ATG16L1; APG16L; Autophagy-related protein 16-1; APG16-like 1); Anti -ATG16L; anti-ATG16L1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ATG16L1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377~407 amino acids from the C-term of human APG16L.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hAPG16L-K366 in NIH-3T3, HepG2, Hela, Jurkat and NCI-H460 cell line lysates (35ug/lane). APG16L (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-ATG16L1 antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is esential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane.

Product Categories/Family for anti-ATG16L1 antibody

Antibodies; Abs to Autophagy

NCBI and Uniprot Product Information

• NCBI GI #: 298228964
• NCBI GeneID: 55054
• NCBI Accession #: NP_942593.2
• NCBI GenBank Nucleotide #: NM_198890.2
• UniProt Accession #: Q676U5; Q6IPN1; Q6UXW4; Q6ZVZ5; Q8NCY2; Q96JV5; Q9H619; A3EXK9; A3EXL0; B6ZDH0
• Molecular Weight: 68,265 Da
• NCBI Official Full Name: autophagy-related protein 16-1 isoform 3
• NCBI Official Synonym Full Names: autophagy related 16-like 1 (S. cerevisiae)
• NCBI Official Symbol: ATG16L1
• NCBI Official Synonym Symbols: IBD10; WDR30; APG16L; ATG16A; ATG16L
• NCBI Protein Information: autophagy-related protein 16-1; APG16L beta; WD repeat domain 30; ATG16 autophagy related 16-like 1
• UniProt Protein Name: Autophagy-related protein 16-1
• Protein Family: Autophagy-related protein
• UniProt Gene Name: ATG16L1
• UniProt Synonym Gene Names: APG16L
• UniProt Entry Name: A16L1_HUMAN

NCBI Description

The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010]

Uniprot Description

Function: Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Ref.11

Subunit structure: Homooligomer. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa)

By similarity. Interacts with RAB33B

By similarity. Interacts with TMEM59, TLR2 and NOD2. Ref.11

Subcellular location: Cytoplasm

By similarity. Preautophagosomal structure membrane; Peripheral membrane protein

By similarity. Note: Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5

By similarity.

Involvement in disease: Inflammatory bowel disease 10 (IBD10) [MIM:611081]: A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.2 Ref.12

Sequence similarities: Belongs to the WD repeat ATG16 family.Contains 7 WD repeats.

Sequence caution: The sequence BAB15448.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.The sequence BAB55412.1 differs from that shown. Reason: Erroneous initiation.