Western Blot (WB)
(Western blot analysis of ATG14 Antibody (Center) in 293 cell line lysates (35ug/lane).ATG14 (arrow) was detected using the purified Pab.)
Rabbit anti-Human ATG14 Polyclonal Antibody | anti-ATG14 antibody
ATG14, ID (ATG14, KIAA0831, Beclin 1-associated autophagy-related key regulator, Autophagy-related protein 14-like protein) (Azide free) (HRP)
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of ATG14 Antibody (Center) in 293 cell line lysates (35ug/lane).ATG14 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ATG14 Antibody (Center) in 293 cell line lysates (35ug/lane).ATG14 (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(ATG14 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ATG14 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
Barkor: Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Forms a complex with BECN1, PIK3C3 and PIK3R4, but not with UVRAG, nor with KIAA0226/Rubicon. UVRAG and ATG14/Barkor form mutually exclusive complexes with BECN1 through direct competition. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. Interacts with PIK3CB. Belongs to the Barkor family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Autophagy
Chromosomal Location of Human Ortholog: 14q22.3
Cellular Component: nucleoplasm; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; phagocytic vesicle; axoneme; nucleus
Molecular Function: protein binding
Biological Process: regulation of protein amino acid phosphorylation; macroautophagy; mitochondrion degradation; endosome to lysosome transport; positive regulation of autophagy; autophagic vacuole formation
Research Articles on ATG14
Similar Products
Product Notes
The ATG14 atg14 (Catalog #AAA6276000) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATG14, ID (ATG14, KIAA0831, Beclin 1-associated autophagy-related key regulator, Autophagy-related protein 14-like protein) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATG14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ATG14 atg14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
