Western Blot (WB)
(ATF7 Antibody (N-term) western blot analysis in huamn placenta tissue lysates (35ug/lane).This demonstrates the ATF7 antibody detected the ATF7 protein (arrow).)
Rabbit anti-Human ATF7 Polyclonal Antibody | anti-ATF7 antibody
ATF7 Antibody (N-term)
Western Blot (WB)
(ATF7 Antibody (N-term) western blot analysis in huamn placenta tissue lysates (35ug/lane).This demonstrates the ATF7 antibody detected the ATF7 protein (arrow).)
Western Blot (WB)
(ATF7 Antibody (N-term) western blot analysis in huamn placenta tissue lysates (35ug/lane).This demonstrates the ATF7 antibody detected the ATF7 protein (arrow).)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with ATF7 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATF7 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ATF7 immunoreactivity is localized to Nucleus and Cytoplasm significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with ATF7 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATF7 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ATF7 immunoreactivity is localized to Nucleus and Cytoplasm significantly.)
Flow Cytometry (FC/FACS)
(ATF7 Antibody (N-term) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ATF7 Antibody (N-term) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
ATF7: Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]- 3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family. Belongs to the bZIP family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: protein binding; enzyme binding; sequence-specific DNA binding; metal ion binding; mitogen-activated protein kinase binding; transcription factor binding; transcription factor activity
Biological Process: viral reproduction; transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter
Research Articles on ATF7
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Product Notes
The ATF7 atf7 (Catalog #AAA9212522) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 68-96. The ATF7 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATF7 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the ATF7 atf7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATF7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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