Western Blot (WB)
(ATF5 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ATF5 antibody detected the ATF5 protein (arrow).)
Rabbit anti-Human ATF5 Polyclonal Antibody | anti-ATF5 antibody
ATF5, CT (ATF5, ATFX, Cyclic AMP-dependent transcription factor ATF-5, Activating transcription factor 5, Transcription factor ATFx) (APC)
Gene Names
ATF5; ATFX; HMFN0395
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ATF5; Polyclonal Antibody; CT (ATF5; ATFX; Cyclic AMP-dependent transcription factor ATF-5; Activating transcription factor 5; Transcription factor ATFx) (APC); Transcription factor ATFx; anti-ATF5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-ATF5 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
ATF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-216 amino acids from the C-terminal region of human ATF5.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(ATF5 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ATF5 antibody detected the ATF5 protein (arrow).)
Western Blot (WB)
(ATF5 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ATF5 antibody detected the ATF5 protein (arrow).)
Product Categories/Family for anti-ATF5 antibody
References
Wei, Y., et al. J. Biochem. 148(2):171-178(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Tomppo, L., et al. Biol. Psychiatry 65(12):1055-1062(2009). Li, G., et al. Mol. Cancer Res. 7(6):933-943(2009). Uekusa, H., et al. Biochem. Biophys. Res. Commun. 380(3):673-678(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,674 Da
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-5
NCBI Official Synonym Full Names
activating transcription factor 5
NCBI Official Symbol
ATF5
NCBI Official Synonym Symbols
ATFX; HMFN0395
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-5; transcription factor ATFx; cAMP-dependent transcription factor ATF-5
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-5
Protein Family
UniProt Gene Name
ATF5
UniProt Synonym Gene Names
ATFX; cAMP-dependent transcription factor ATF-5
UniProt Entry Name
ATF5_HUMAN
Uniprot Description
ATF-5: Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Belongs to the bZIP family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm
Molecular Function: protein binding; heat shock protein binding; transcription corepressor activity
Biological Process: circadian rhythm; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; olfactory bulb interneuron development; multicellular organism growth; negative regulation of astrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of apoptosis; post-embryonic development
Research Articles on ATF5
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Product Notes
The ATF5 atf5 (Catalog #AAA6275887) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATF5, CT (ATF5, ATFX, Cyclic AMP-dependent transcription factor ATF-5, Activating transcription factor 5, Transcription factor ATFx) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATF5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ATF5 atf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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