Western Blot (WB)
((1.5ug/ml) staining of Human Heart lyssate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat ATF5 Polyclonal Antibody | anti-ATF5 antibody
Goat anti-ATF5 Antibody
Western Blot: Approx 29kDa band observed in Human Heart lysates (calculated MW of 30.7kDa according to NP_036200.2). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1.5ug/ml) staining of Human Heart lyssate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1.5ug/ml) staining of Human Heart lyssate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
Uniprot Description
ATF-5: Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Belongs to the bZIP family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: centrosome; cytoplasm; nucleoplasm; nucleus; transcription factor complex
Molecular Function: chromatin binding; heat shock protein binding; kinase binding; protein binding; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity; tubulin binding
Biological Process: circadian rhythm; fat cell differentiation; granule cell precursor proliferation; multicellular organism growth; negative regulation of apoptosis; negative regulation of astrocyte differentiation; negative regulation of cell proliferation; negative regulation of transcription, DNA-dependent; olfactory bulb interneuron development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; post-embryonic development; regulation of centrosome cycle; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter
Research Articles on ATF5
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Product Notes
The ATF5 atf5 (Catalog #AAA422175) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ATF5 Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATF5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 29kDa band observed in Human Heart lysates (calculated MW of 30.7kDa according to NP_036200.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the ATF5 atf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EVYKARSQRT RSC. It is sometimes possible for the material contained within the vial of "ATF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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