Rabbit anti-Human ATF3 Polyclonal Antibody | anti-ATF3 antibody

ATF3, CT (ATF3, Cyclic AMP-dependent transcription factor ATF-3, Activating transcription factor 3) (Biotin)

Cat. Num. AAA6275855

• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ATF3; Polyclonal Antibody; CT (ATF3; Cyclic AMP-dependent transcription factor ATF-3; Activating transcription factor 3) (Biotin); Activating transcription factor 3; anti-ATF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ATF3.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ATF3 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 140-168 in the C-terminal region of human ATF3. Species sequence homology: bovine, mouse, rat
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS6008065. This demonstrates that MBS6008065 detected the ATF3 protein (arrow).)

Western Blot (WB)

(Western Blot analysis of ATF3 (arrow) in 293 cell lysates (2 ug/lane) using MBS6008065 either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATF3 gene.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of CEM cells using MBS6008065 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ATF3 antibody

Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.

Product Categories/Family for anti-ATF3 antibody

Antibodies; Transcription Factors

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Lee, S.H., et al. Oncogene 29(37):5182-5192(2010). 3. Park, H.J., et al. Biochem. Biophys. Res. Commun. 400(1):72-77(2010). 4. Wu, X., et al. Nature 465(7296):368-372(2010). 5. Koh, I.U., et al. FEBS J. 277(10):2304-2317(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 71902536
• NCBI GeneID: 467
• NCBI Accession #: NP_001025458.1; NP_001665.1
• NCBI GenBank Nucleotide #: NM_001030287.3
• UniProt Accession #: P18847; Q6ICQ9; Q7Z566; Q8WYM6
• Molecular Weight: 20,576 Da
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-3 isoform 1
• NCBI Official Synonym Full Names: activating transcription factor 3
• NCBI Official Symbol: ATF3
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-3
• Protein Family: Activating transcription factor
• UniProt Gene Name: ATF3
• UniProt Synonym Gene Names: cAMP-dependent transcription factor ATF-3
• UniProt Entry Name: ATF3_HUMAN

NCBI Description

This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]

Uniprot Description

ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; transcription corepressor activity; transcription factor activity

Biological Process: cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; transcription, DNA-dependent; unfolded protein response; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; gluconeogenesis