NAGA recombinant protein

NAGA, 18-411aa, Human, His tag, Baculovirus

Cat. Num. AAA206383

• Gene Names: NAGA; GALB; D22S674
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: NAGA; 18-411aa; Human; His tag; Baculovirus; Alpha-N-acetylgalactosaminidase; D22S674; GALB; Acetylgalactosaminidase; alpha N (alpha galactosidase B); Alpha galactosidase B; Alpha N acetylgalactosaminidase; N acetylgalactosaminidase; alpha; NAGA recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: LDNGLLQTPP MGWLAWERFR CNINCDEDPK NCISEQLFME MADRMAQDGW RDMGYTYLNI DDCWIGGRDA SGRLMPDPKR FPHGIPFLAD YVHSLGLKLG IYADMGNFTC MGYPGTTLDK VVQDAQTFAE WKVDMLKLDG CFSTPEERAQ GYPKMAAALN ATGRPIAFSC SWPAYEGGLP PRVNYSLLAD ICNLWRNYDD IQDSWWSVLS ILNWFVEHQD ILQPVAGPGH WNDPDMLLIG NFGLSLEQSR AQMALWTVLA APLLMSTDLR TISAQNMDIL QNPLMIKINQ DPLGIQGRRI HKEKSLIEVY MRPLSNKASA LVFFSCRTDM PYRYHSSLGQ LNFTGSVIYE AQDVYSGDII SGLRDETNFT VIINPSGVVM WYLYPIKNLE MSQQHHHHHH
• Sequence Length: 411

• Applicable Applications for NAGA recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for NAGA recombinant protein

NAGA, also known as alpha-N-acetylgalactosaminidase, is a lysosomal exoglycosidase that cleaves terminal alpha-N-acetylgalactosamine residues from glycopeptides and glycolipids. It is hardly expected to cause an allergic reaction in Fabry disease patients. It is highly promising as a new and safe enzyme for ERT for Fabry disease. Recombinant human NAGA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for NAGA recombinant protein

Metabolism; Signal Transduction

References

Clark NE., et al. (2009) J Mol Biol. 393:435-447.; Tajima Y., et al. (2009) Am J Hum Genet. 85:569-580.

NCBI and Uniprot Product Information

• NCBI GI #: 4557781
• NCBI GeneID: 4668
• NCBI Accession #: NP_000253
• NCBI GenBank Nucleotide #: NM_000262.2
• UniProt Accession #: P17050
• Molecular Weight: 45.5kDa (400aa) 40-57kDa (SDS-PAGE under reducing conditions
• NCBI Official Full Name: alpha-N-acetylgalactosaminidase
• NCBI Official Synonym Full Names: alpha-N-acetylgalactosaminidase
• NCBI Official Symbol: NAGA
• NCBI Official Synonym Symbols: GALB; D22S674
• NCBI Protein Information: alpha-N-acetylgalactosaminidase
• UniProt Protein Name: Alpha-N-acetylgalactosaminidase
• Protein Family: Alpha-N-acetylgalactosaminidase
• UniProt Gene Name: NAGA

NCBI Description

NAGA encodes the lysosomal enzyme alpha-N-acetylgalactosaminidase, which cleaves alpha-N-acetylgalactosaminyl moieties from glycoconjugates. Mutations in NAGA have been identified as the cause of Schindler disease types I and II (type II also known as Kanzaki disease). [provided by RefSeq, Jul 2008]

Uniprot Description

Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids.