Rabbit anti-Human ATF3 Polyclonal Antibody | anti-ATF3 antibody

Anti-Human ATF3 Antibody

Cat. Num. AAA4156744

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity Purified
• Synonyms: ATF3; Polyclonal Antibody; Anti-Human ATF3 Antibody; Human ATF3; anti-ATF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This affinity-purified antibody is directed against human ATF3 and is useful in determining its presence in various assays including ELISA and western blotting. This polyclonal antibody recognizes over-expressed ATF3 protein found in various expression systems. Reactivity is observed against human ATF3. Cross reactivity with ATF3 from other mammalian sources has not been tested.
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 181

• Applicable Applications for anti-ATF3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Affinity purified anti-ATF3 has been tested by ELISA and western blotting against recombinant forms of the protein. Although not tested, this antibody is likely function in most immunoassays including immunofluorescence microscopy, immunohistochemistry.
• Immunogen: This antibody was produced from a synthetic peptide corresponding to aa 113-130 of human ATF3.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Related Product Information for anti-ATF3 antibody

ATF3, or Activating Transcription Factor 3, is a member of mammalian activation TF/CREB protein family of transcription factors. ATF3 binds the cAMP response element (cre) (consensus: 5'-gtgacgt[ac][ag]-3'), a sequence present in many viral and cellular promoters. However, ATF3 represses rather than activates transcription from promoters with ATF sites stabilizing inhibitory co-factors at the promoter. Alternate splicing forms of ATF3, called ATF3 delta Zip, lack the leucine zipper domain and do not bind DNA. ATF3 delta Zip stimulates transcription, presumably by sequestering inhibitory co-factors away from the promoter. Human ATF3 (SwissProt 18847) is a 20575 Da protein composed of 181 amino acids.

NCBI and Uniprot Product Information

• NCBI GI #: 47496615
• NCBI GeneID: 467
• NCBI Official Full Name: ATF3, partial
• NCBI Official Synonym Full Names: activating transcription factor 3
• NCBI Official Symbol: ATF3
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-3
• Protein Family: Activating transcription factor

NCBI Description

This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]