ATF3 recombinant protein

ATF3, 1-181aa, Human, E Coli (Denatured)

Cat. Num. AAA204291

• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: ATF3; 1-181aa; Human; E Coli (Denatured); Activating transcription factor 3; ATF3deltaZip2; ATF3deltaZip2c; ATF3deltaZip3; cAMP dependent transcription factor ATF3; Cyclic AMP dependent transcription factor ATF3; Cyclic AMP-dependent transcription factor ATF-3; ATF3 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl, pH8.0, 2M urea, 20%glycerol
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MMLQHPGQVS ASEVSASAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ NKHLCHRMSS ALESVTVSDR PLGVSITKAE VAPEEDERKK RRRERNKIAA AKCRNKKKEK TECLQKESEK LESVNAELKA QIEELKNEKQ HLIYMLNLHR PTCIVRAQNG RTPEDERNLF IQQIKEGTLQ S
• Sequence Length: 181

• Applicable Applications for ATF3 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ATF3 recombinant protein

ATF3 (activating transcription factor 3) is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription, it is presumed, by sequestering inhibitory co-factors away from the promoter.

Product Categories/Family for ATF3 recombinant protein

Signal Transduction

References

Lv D., et al. (2011) IuBMB Life. 63 (1):62-9. ; Chen BP., et al. (1994) J Biol Chem 269 (22): 15819-26.

NCBI and Uniprot Product Information

• NCBI GI #: 4502263
• NCBI GeneID: 467
• NCBI Accession #: NP_001665
• NCBI GenBank Nucleotide #: NM_001674.3
• UniProt Accession #: P18847; Q5VTZ2; Q6ICQ9; Q7Z566; Q8WYM6
• Molecular Weight: 20.6kDa (181aa)
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-3 isoform 1
• NCBI Official Synonym Full Names: activating transcription factor 3
• NCBI Official Symbol: ATF3
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-3
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-3
• Protein Family: Activating transcription factor
• UniProt Gene Name: ATF3
• UniProt Synonym Gene Names: cAMP-dependent transcription factor ATF-3

NCBI Description

This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]

Uniprot Description

This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.