Asialoglycoprotein Receptor Recombinant Protein | ASGR1 recombinant protein

Asialoglycoprotein Receptor Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120477

• Gene Names: ASGR1; HL-1; ASGPR; ASGPR1; CLEC4H1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Asialoglycoprotein Receptor; Asialoglycoprotein Receptor Protein; Human; Recombinant (His Tag); Human ASGPR1/ASGR1 Protein (His Tag); ASGPR Protein; ASGPR1 Protein; CLEC4H1 Protein; HL-1 Protein; asialoglycoprotein receptor 1; ASGR1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln62-Leu291

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human ASGR1 isoform 1 (P07306)(Gln 62-Leu 291) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ASGR1 recombinant protein

Background: The asialoglycoprotein receptor (ASGPR), an endocytotic cell surface receptor expressed by hepatocytes, is triggered by triantennary binding to galactose residues of macromolecules such as asialoorosomucoid (ASOR). ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42kDa glycoprotein ASGPR1(MHL-1) and a minor 51kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The research regarded that ASGPR1 could be targeted for anti- hepatitis B virus (HBV) drug development.

References

Yang J, et al. (2006) Antisense oligonucleotides targeted against asialoglycoprotein receptor 1 block human hepatitis B virus replication. J Viral Hepat. 13(3): 158-65.Li Y, et al. (2008) Targeted delivery of macromolecular drugs: asialoglycoprotein receptor (ASGPR) expression by selected hepatoma cell lines used in antiviral drug development. Curr Drug Deliv. 5(4): 299-302.

NCBI and Uniprot Product Information

• NCBI GI #: 308387363
• NCBI GeneID: 432
• NCBI Accession #: NP_001184145.1
• NCBI GenBank Nucleotide #: NM_001197216.2
• UniProt Accession #: P07306; I3L1X1
• Molecular Weight: 33,186 Da
• NCBI Official Full Name: asialoglycoprotein receptor 1 isoform b
• NCBI Official Synonym Full Names: asialoglycoprotein receptor 1
• NCBI Official Symbol: ASGR1
• NCBI Official Synonym Symbols: HL-1; ASGPR; ASGPR1; CLEC4H1
• NCBI Protein Information: asialoglycoprotein receptor 1; hepatic lectin H1; C-type lectin domain family 4 member H1
• UniProt Protein Name: Asialoglycoprotein receptor 1
• Protein Family: Asialoglycoprotein receptor
• UniProt Gene Name: ASGR1
• UniProt Synonym Gene Names: CLEC4H1; ASGP-R 1; ASGPR 1; HL-1
• UniProt Entry Name: ASGR1_HUMAN

NCBI Description

This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the more abundant major subunit. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

ASGR1: Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: integral to plasma membrane; extracellular region

Molecular Function: protein binding; protein homodimerization activity; metal ion binding; asialoglycoprotein receptor activity; carbohydrate binding

Biological Process: receptor-mediated endocytosis; cellular response to extracellular stimulus