Western Blot (WB)
(ASAP3 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ASAP3 antibody detected the ASAP3 protein (arrow).)
Rabbit ASAP3 Polyclonal Antibody | anti-ASAP3 antibody
ASAP3, NT (ASAP3, DDEFL1, UPLC1, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3, Development and differentiation-enhancing factor-like 1, Protein up-regulated in liver cancer 1) (PE)
Gene Names
ASAP3; ACAP4; UPLC1; CENTB6; DDEFL1
Reactivity
Hamster, Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ASAP3; Polyclonal Antibody; NT (ASAP3; DDEFL1; UPLC1; Arf-GAP with SH3 domain; ANK repeat and PH domain-containing protein 3; Development and differentiation-enhancing factor-like 1; Protein up-regulated in liver cancer 1) (PE); Protein up-regulated in liver cancer 1; anti-ASAP3 antibody
Host
Rabbit
Reactivity
Hamster, Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-ASAP3 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ASAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human ASAP3.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(ASAP3 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ASAP3 antibody detected the ASAP3 protein (arrow).)
Western Blot (WB)
(ASAP3 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ASAP3 antibody detected the ASAP3 protein (arrow).)
Western Blot (WB)
(ASAP3 Antibody (N-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the ASAP3 antibody detected the ASAP3 protein (arrow).)
Western Blot (WB)
(ASAP3 Antibody (N-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the ASAP3 antibody detected the ASAP3 protein (arrow).)
Immunohistochemistry (IHC)
(ASAP3 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ASAP3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ASAP3 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ASAP3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-ASAP3 antibody
This gene encodes a member of a subfamily of ADP-ribosylation factor(Arf) GTPase-activating proteins that contain additional ankyrin repeat and pleckstrin homology domains. The Arf GAP domain of this protein catalyzes the hydrolysis of GTP bound to Arf proteins. The encoded protein promotes cell differentiation and migration and has been implicated in cancer cell invasion. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-ASAP3 antibody
References
Ismail, S.A., et al. Cell 141(5):812-821(2010). Ha, V.L., et al. J. Biol. Chem. 283(22):14915-14926(2008). Fang, Z., et al. Mol. Cell Proteomics 5(8):1437-1449(2006). Okabe, H., et al. Int. J. Oncol. 24(1):43-48(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
99,155 Da
NCBI Official Full Name
arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3 isoform b
NCBI Official Synonym Full Names
ArfGAP with SH3 domain, ankyrin repeat and PH domain 3
NCBI Official Symbol
ASAP3
NCBI Official Synonym Symbols
ACAP4; UPLC1; CENTB6; DDEFL1
NCBI Protein Information
arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3; centaurin, beta 6; ARF6 GTPase-activating protein; protein up-regulated in liver cancer 1; up-regulated in liver cancer 1 (UPLC1); development and differentiation enhancing factor-lik
UniProt Protein Name
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3
UniProt Gene Name
ASAP3
UniProt Synonym Gene Names
DDEFL1; UPLC1
UniProt Entry Name
ASAP3_HUMAN
NCBI Description
This gene encodes a member of a subfamily of ADP-ribosylation factor(Arf) GTPase-activating proteins that contain additional ankyrin repeat and pleckstrin homology domains. The Arf GAP domain of this protein catalyzes the hydrolysis of GTP bound to Arf proteins. The encoded protein promotes cell differentiation and migration and has been implicated in cancer cell invasion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]
Research Articles on ASAP3
Similar Products
Product Notes
The ASAP3 asap3 (Catalog #AAA6275511) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ASAP3, NT (ASAP3, DDEFL1, UPLC1, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3, Development and differentiation-enhancing factor-like 1, Protein up-regulated in liver cancer 1) (PE) reacts with Hamster, Human and may cross-react with other species as described in the data sheet. AAA Biotech's ASAP3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-100 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ASAP3 asap3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASAP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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