Rabbit anti-Human Aryl Hydrocarbon Receptor Polyclonal Antibody | anti-AHR antibody

Anti-Aryl Hydrocarbon Receptor Antibody, Rabbit Polyclonal

Cat. Num. AAA8118912

• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: Aryl Hydrocarbon Receptor; Polyclonal Antibody; Anti-Aryl Hydrocarbon Receptor Antibody; Rabbit Polyclonal; Aryl Hydrocarbon Receptor Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-bHLHe76 Antibody; aryl hydrocarbon receptor; anti-AHR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Aryl Hydrocarbon Receptor
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid. 0.2um filtered solution in PBS

• Applicable Applications for anti-AHR antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:250-1:1000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human Aryl Hydrocarbon Receptor
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Aryl Hydrocarbon Receptor, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Immunofluorescence (IF)

(Immunofluorescence staining of AHR in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human AHR polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor-594-conjugated Goat.)

Immunohistochemistry (IHC)

(Immunochemical staining of human AHR in human colon with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human AHR in human breast carcinoma with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-AHR antibody

Background: The aryl hydrocarbon receptor (AHR) is a cytosolic ligand-activated transcription factor involved in xenobiotic sensing, cell cycle regulation and cell development. Aryl hydrocarbon receptor (AHR) is a chemical sensor that is expressed abundantly in epidermal keratinocytes. Oxidative AHR ligands induce the production of reactive oxygen species. However, antioxidant AHR ligands inhibit reactive oxygen species generation via activation of nuclear factor-erythroid 2-related factor-2, which is a master switch for antioxidative signalling. In addition, AHR signalling accelerates epidermal terminal differentiation, but excessive acceleration by oxidative ligands, such as dioxins, may induce chloracne and inflammation. However, antioxidative phytochemical ligands induce the beneficial acceleration of epidermal differentiation that repairs skin barrier disruption. Aryl hydrocarbon receptor (AHR) is a ligand activated transcription factor and involved in tumorigenesis of many cancers. To sum-up we found that AHR signalling pathway components were upregulated, as the grade of the meningioma progresses from low to high grade, suggesting an important role of AHR signalling pathway in human meningioma. Aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor, connecting environmental stimulators with the immune system. AHR had been demonstrated an anti-inflammatory role in macrophages, which suggest that decreased AHR expression is associated with pro-inflammatory M1 macrophage and BD susceptibility.