Rabbit Arrestin beta1 Polyclonal Antibody | anti-ARRB1 antibody

Arrestin beta1 Polyclonal Antibody

Cat. Num. AAA3012262

• Gene Names: ARRB1; ARB1; ARR1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide affinity purified
• Synonyms: Arrestin beta1; Polyclonal Antibody; Arrestin beta1 Polyclonal Antibody; ARRB1; ARR1; ARB1; Arrestin 2; Arrestin beta 1; Arrestin beta-1; Beta-arrestin-1; anti-ARRB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Arrestin beta1 polyclonal antibody detects endogenous levels of Arrestin beta1 protein.
• Purity/Purification: Peptide affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 418

• Applicable Applications for anti-ARRB1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:1000; ICC: 1:50-1:200; IHC: 1:50-1:100; FC/FACS: 1:50-1:100
• Immunogen: Peptide
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of beta Arrestin 1 on human placenta tissue lysate using anti-beta Arrestin 1 antibody at 1/500 dilution.)

Immunocytochemistry (ICC)

(ICC staining beta Arrestin 1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-ARRB1 antibody

The members of the G protein coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. Members of the beta-Arrestin family regulate receptor binding to G proteins. beta-Arrestins have been found to be located at postsynaptic sites, where they are thought to act in concert with betaARK (betaARK1, also designated GRK 2, or betaARK2, also designated GRK 3) to regulate G protein-coupled neurotransmitter receptors. Expression of beta-Arrestin-1 and b-Arrestin-2 is seen predominantly in spleen and neuronal tissues. It has been shown that beta-Arrestin-1 expression is modulated by intracellular cAMP, which may be a novel mechanism for the regulation of receptor-mediated responses.

NCBI and Uniprot Product Information

• NCBI GI #: 10880136
• NCBI GeneID: 408
• NCBI Accession #: NP_004032.2
• NCBI GenBank Nucleotide #: NP_004032.2
• UniProt Accession #: P49407; Q8BWG8; P29066
• Molecular Weight: 50kDa
• NCBI Official Full Name: beta-arrestin-1 isoform A
• NCBI Official Synonym Full Names: arrestin beta 1
• NCBI Official Symbol: ARRB1
• NCBI Official Synonym Symbols: ARB1; ARR1
• NCBI Protein Information: beta-arrestin-1
• UniProt Protein Name: Beta-arrestin-1
• Protein Family: Beta-arrestin
• UniProt Gene Name: ARRB1
• UniProt Synonym Gene Names: ARR1
• UniProt Entry Name: ARRB1_HUMAN

NCBI Description

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011]

Uniprot Description

ARRB1: regulates G-protein coupled receptors (GPCR) signaling by mediating both receptor desensitization and resensitization processes. Binds to GRK-phosphorylated receptor and sterically preclude its coupling to the cognate G- protein; the binding appears to require receptor determinants exposed only in the active receptor conformation. Targets many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins). Internalized arrestin-receptor complexes traffic to intracellular endosomes, where they remain uncoupled from G-proteins. Two different modes of arrestin-mediated internalization occur. Beta-arrestins function as multivalent adapter proteins that can switch the GPCR from a G-protein signaling mode that transmits short-lived signals from the plasma membrane via small molecule second messengers and ion channels to a beta-arrestin signaling mode that transmits a distinct set of signals that are initiated as the receptor internalizes and transits the intracellular compartment. Also involved in regulation of receptors other than GPCRs. Involved in Toll-like receptor and IL-1 receptor signaling through the interaction with TRAF6 which prevents TRAF6 autoubiquitination and oligomerization required for activation of NF-kappa-B and JUN. Binds phosphoinositides. Binds inositolhexakisphosphate (InsP6). Involved in IL8-mediated granule release in neutrophils. Interacts with phosphorylated ADRB2 and CHRM2. Interacts with SRC (via the SH3 domain and the protein kinase domain); the interaction is independent of the phosphorylation state of SRC C-terminus. Interacts with RAF1, CHUK, IKBKB and Nik. Interacts with DVL1 and DVL2; the interaction is enhanced by DVL phosphorylation. Interacts with IGF1R. Belongs to the arrestin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; Golgi membrane; cytoplasmic vesicle membrane; lysosomal membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex; coated pit; cytoplasmic vesicle; pseudopodium; cytosol; nucleus; chromatin

Molecular Function: angiotensin receptor binding; enzyme inhibitor activity; insulin-like growth factor receptor binding; protein binding; histone acetyltransferase activity; mitogen-activated protein kinase kinase binding; ubiquitin protein ligase binding; caspase inhibitor activity; transcription factor binding; GTPase activator activity

Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; platelet activation; Notch signaling pathway; positive regulation of protein binding; positive regulation of receptor internalization; positive regulation of Rho protein signal transduction; protein ubiquitination; negative regulation of caspase activity; positive regulation of peptidyl-serine phosphorylation; protein transport; G-protein coupled receptor internalization; inhibition of NF-kappaB transcription factor; negative regulation of interleukin-8 production; negative regulation of interleukin-6 production; phototransduction; stress fiber formation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein ubiquitination; positive regulation of histone acetylation; post-Golgi vesicle-mediated transport; positive regulation of protein amino acid phosphorylation; blood coagulation; positive regulation of GTPase activity