Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human ARRb2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Arrestin Beta 2 (ARRb2) Polyclonal Antibody | anti-ARRb2 antibody
Polyclonal Antibody to Arrestin Beta 2 (ARRb2)
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-DICLFSTAQ YKCPVAQLEQ DDQVSPSSTF CKVYTITPLLSDNREKRGLA LDGKLKHEDT NLASSTIVKE GANKEVLGIL VSYRVKVKLV VSRGGDVSVELPFVLMHPKP HDHIPLPRPQ SAAPETDVPV DTNLIEFDTN YATDDDIVFE DFARLRLKGMKDDDYDDQLC
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human ARRb2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human ARRb2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
NCBI and Uniprot Product Information
NCBI Description
Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]
Uniprot Description
ARRB2: a member of the arrestin/beta-arrestin protein family. These proteins participate in agonist-mediated desensitization of G-protein-coupled receptors. Acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Expressed at high levels in the CNS and peripheral blood leukocytes.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 17p13.2
Cellular Component: basolateral plasma membrane; clathrin-coated pit; cytoplasm; cytoplasmic vesicle; cytosol; dendritic spine; endocytic vesicle; intracellular membrane-bound organelle; nucleus; plasma membrane; postsynaptic density; postsynaptic membrane
Molecular Function: 14-3-3 protein binding; alpha-1A adrenergic receptor binding; alpha-1B adrenergic receptor binding; angiotensin receptor binding; D1 dopamine receptor binding; enzyme binding; follicle-stimulating hormone receptor binding; G-protein coupled receptor binding; identical protein binding; mitogen-activated protein kinase binding; platelet activating factor receptor binding; protein binding; protein complex binding; protein complex scaffold activity; protein domain specific binding; protein kinase B binding; receptor binding; type 1 angiotensin receptor binding; type 2A serotonin receptor binding; ubiquitin protein ligase binding
Biological Process: adult walking behavior; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; brain development; detection of temperature stimulus involved in sensory perception of pain; dopamine receptor signaling pathway; follicle-stimulating hormone signaling pathway; G-protein coupled receptor internalization; G-protein coupled receptor signaling pathway; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; negative regulation of interleukin-1 beta production; negative regulation of interleukin-12 production; negative regulation of interleukin-6 production; negative regulation of natural killer cell mediated cytotoxicity; negative regulation of NF-kappaB transcription factor activity; negative regulation of protein kinase B signaling cascade; negative regulation of protein phosphorylation; negative regulation of protein ubiquitination; negative regulation of release of cytochrome c from mitochondria; negative regulation of smooth muscle cell apoptotic process; negative regulation of toll-like receptor signaling pathway; negative regulation of tumor necrosis factor production; platelet activation; positive regulation of calcium ion transport; positive regulation of cardiac muscle cell differentiation; positive regulation of DNA biosynthetic process; positive regulation of ERK1 and ERK2 cascade; positive regulation of gene expression; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase B signaling; positive regulation of protein ubiquitination; positive regulation of receptor internalization; positive regulation of synaptic transmission, dopaminergic; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; protein transport; protein ubiquitination; receptor internalization; transcription from RNA polymerase II promoter; transforming growth factor beta receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway
Research Articles on ARRb2
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Product Notes
The ARRb2 arrb2 (Catalog #AAA2004022) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Arrestin Beta 2 (ARRb2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Arrestin Beta 2 (ARRb2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ARRb2 arrb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -DICLFSTAQ YKCPVAQLEQ DDQVSPSSTF CKVYTITPLL SDNREKRGLA LDGKLKHEDT NLASSTIVKE GANKEVLGIL VSYRVKVKLV VSRGGDVSVE LPFVLMHPKP HDHIPLPRPQ SAAPETDVPV DTNLIEFDTN YATDDDIVFE DFARLRLKGM KDDDYDDQLC. It is sometimes possible for the material contained within the vial of "Arrestin Beta 2 (ARRb2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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