FLISA
(FLISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-CY5 .)
IgG secondary antibody
Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-CY5
Reactivity
Goat
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Affinity chromatography on goat IgG covalently linked to agarose
Synonyms
IgG; Polyclonal Secondary Antibody; Donkey Anti-Goat IgG(H+L); Mouse/Rat SP ads-CY5; IgG secondary antibody
Host
Donkey
Reactivity
Goat
Clonality
Polyclonal
Isotype
Donkey IgG
Specificity
Reacts with the heavy and light chains of goat IgG
Purity/Purification
Affinity chromatography on goat IgG covalently linked to agarose
Form/Format
Phosphate buffered saline containing <0.1% sodium azide
Concentration
1.0mg/mL (varies by lot)
Applicable Applications for IgG secondary antibody
Flow Cytometry (FC/FACS), Immunohistochemistry-Frozen (IHC-F) Sections, Immunohistochemistry-Paraffin (IHC-P) Sections, Western Blot (WB), Multiplex
Application Notes
Quality tested applications for relevant formats include-
ELISA 1 FLISA
Other referenced applications for relevant formats include-
Flow Cytometry 2, 3
Immunohistochemistry-Frozen Sections 4
Immunohistochemistry-Paraffin Sections 11
Western Blot 5-9
Multiplex 10
ELISA 1 FLISA
Other referenced applications for relevant formats include-
Flow Cytometry 2, 3
Immunohistochemistry-Frozen Sections 4
Immunohistochemistry-Paraffin Sections 11
Western Blot 5-9
Multiplex 10
Source
Pooled antisera from donkeys hyperimmunized with goat IgG
Conjugation
CY5 (Cyanine 5)
Cross Adsorption
Mouse and rat serum proteins (SP); may react with immunoglobulins from other species and the light chains of other goat immunoglobulins
Preparation and Storage
Store at 2-8 degree C. Avoid exposure to light. Please refer to product specific SDS
FLISA
(FLISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-CY5 .)
FLISA
(FLISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-CY5 .)
References
1. Migliore MM, Ortiz R, Dye S, Campbell RB, Amiji MM, Waszczak BL. Neurotrophic and neuroprotective efficacy of intranasal GDNF in a rat model of Parkinson's disease. Neuroscience. 2014;274:11-23. (ELISA)
2. Rasche SS, Phillips M, McInerney MF, Sercarz EE, Quinn A. IL-13Ralpha1 expression on beta-cell-specific T cells in NOD mice. Diabetes. 2011;60:1716-25. (FC)
3. Biedro? R, Konopi?ski MK, Marcinkiewicz J, Józefowski S. Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages. PLoS One. 2015;10(4) :e0123293. (FC)
4. Xia RH, Yosef N, Ubogu EE. Selective expression and cellular localization of pro-inflammatory chemokine ligand/receptor pairs in the sciatic nerves of a severe murine experimental autoimmune neuritis model of Guillain-Barré syndrome. Neuropathol Appl Neurobiol. 2010;36:388-98. (IHC-FS)
5. Liu S, Bubar MJ, Lanfranco MF, Hillman GR, Cunningham KA. Serotonin2C receptor localization in GABA neurons of the rat medial prefrontal cortex: implications for understanding the neurobiology of addiction. Neuroscience. 2007;146:1677-88. (WB)
6. Kumar GR, Shum L, Glaunsinger BA. Importin alpha-mediated nuclear import of cytoplasmic poly(A) binding protein occurs as a direct consequence of cytoplasmic mRNA depletion. Mol Cell Biol. 2011;31:3113-25. (WB)
7. Gao H, Yallampalli U, Yallampalli C. Maternal protein restriction reduces expression of angiotensin I-converting enzyme 2 in rat placental labyrinth zone in late pregnancy. Biol Reprod. 2011;86:31. (WB)
8. Gao H, Yallampalli U, Yallampalli C. Protein restriction to pregnant rats increases the plasma levels of angiotensin II and expression of angiotensin II receptors in uterine arteries. Biol Reprod. 2012;86:68. (WB)
9. Bouchez I, Pouteaux M, Canonge M, Genet M, Chardot T, Guillot A, et al. Regulation of lipid droplet dynamics in Saccharomyces cerevisiae depends on the Rab7-like Ypt7p, HOPS complex and V1-ATPase. Biol Open. 2015;00:pii: bio.20148615. (WB)
10. Yang G, Holl TM, Liu Y, Li Y, Lu X, Nicely NI, et al. Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies. J Exp Med. 2013;210:241-56. (Multiplex)
11. SouthernBiotech published data (IHC-PS)
2. Rasche SS, Phillips M, McInerney MF, Sercarz EE, Quinn A. IL-13Ralpha1 expression on beta-cell-specific T cells in NOD mice. Diabetes. 2011;60:1716-25. (FC)
3. Biedro? R, Konopi?ski MK, Marcinkiewicz J, Józefowski S. Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages. PLoS One. 2015;10(4) :e0123293. (FC)
4. Xia RH, Yosef N, Ubogu EE. Selective expression and cellular localization of pro-inflammatory chemokine ligand/receptor pairs in the sciatic nerves of a severe murine experimental autoimmune neuritis model of Guillain-Barré syndrome. Neuropathol Appl Neurobiol. 2010;36:388-98. (IHC-FS)
5. Liu S, Bubar MJ, Lanfranco MF, Hillman GR, Cunningham KA. Serotonin2C receptor localization in GABA neurons of the rat medial prefrontal cortex: implications for understanding the neurobiology of addiction. Neuroscience. 2007;146:1677-88. (WB)
6. Kumar GR, Shum L, Glaunsinger BA. Importin alpha-mediated nuclear import of cytoplasmic poly(A) binding protein occurs as a direct consequence of cytoplasmic mRNA depletion. Mol Cell Biol. 2011;31:3113-25. (WB)
7. Gao H, Yallampalli U, Yallampalli C. Maternal protein restriction reduces expression of angiotensin I-converting enzyme 2 in rat placental labyrinth zone in late pregnancy. Biol Reprod. 2011;86:31. (WB)
8. Gao H, Yallampalli U, Yallampalli C. Protein restriction to pregnant rats increases the plasma levels of angiotensin II and expression of angiotensin II receptors in uterine arteries. Biol Reprod. 2012;86:68. (WB)
9. Bouchez I, Pouteaux M, Canonge M, Genet M, Chardot T, Guillot A, et al. Regulation of lipid droplet dynamics in Saccharomyces cerevisiae depends on the Rab7-like Ypt7p, HOPS complex and V1-ATPase. Biol Open. 2015;00:pii: bio.20148615. (WB)
10. Yang G, Holl TM, Liu Y, Li Y, Lu X, Nicely NI, et al. Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies. J Exp Med. 2013;210:241-56. (Multiplex)
11. SouthernBiotech published data (IHC-PS)
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Product Notes
The IgG (Catalog #AAA679420) is a Secondary Antibody produced from Donkey and is intended for research purposes only. The product is available for immediate purchase. The Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-CY5 reacts with Goat and may cross-react with other species as described in the data sheet. AAA Biotech's IgG can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry-Frozen (IHC-F) Sections, Immunohistochemistry-Paraffin (IHC-P) Sections, Western Blot (WB), Multiplex. Quality tested applications for relevant formats include- ELISA 1 FLISA Other referenced applications for relevant formats include- Flow Cytometry 2, 3 Immunohistochemistry-Frozen Sections 4 Immunohistochemistry-Paraffin Sections 11 Western Blot 5-9 Multiplex 10. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG, Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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