Immunofluorescence (IF)
(MBS9605327 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit ARPP21 Polyclonal Antibody | anti-ARPP21 antibody
ARPP21 Antibody
Gene Names
ARPP21; RCS; TARPP; R3HDM3; ARPP-21
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ARPP21; Polyclonal Antibody; ARPP21 Antibody; 0710001E13Rik; AI853636; ARP21_HUMAN; ARPP-21; cAMP regulated phosphoprotein; 21kDa; cAMP-regulated phosphoprotein 21; cAMP-regulated phosphoprotein; 21-KD; Cyclic AMP-regulated phosphoprotein; 21; D9Bwg1012e; FLJ32997; MGC109368; OTTHUMP00000161358; OTTHUMP00000208635; OTTHUMP00000208636; OTTHUMP00000208637; OTTHUMP00000208638; OTTHUMP00000208639; OTTHUMP00000208640; OTTHUMP00000208641; OTTHUMP00000208646; OTTHUMP00000208648; OTTHUMP00000208651; OTTHUMP00000208652; OTTHUMP00000208653; OTTHUMP00000208657; Ppp1r1c; Ppp1r1cl; R3H domain containing 3; R3HDM3; RCS; Regulator of calmodulin signaling; RGD1307208; RGD1307215; Tarpp; Thymocyte cAMP-regulated phosphoprotein; anti-ARPP21 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ARPP21 antibody detects endogenous levels of total ARPP21
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
89
Applicable Applications for anti-ARPP21 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Tissue Specificity
Isoform 2 is expressed in brain. Isoform 1 is present in immature thymocytes (at protein level).
Predicted Cross Reactivity
Pig, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9605327 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9605327 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9605327 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9605327 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ARPP21 antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ARPP21 antibody.The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-ARPP21 antibody
Function: Isoform 2 may act as a competitive inhibitor of calmodulin-dependent enzymes such as calcineurin in neurons.
Subunit Structure: Interacts with CALM1.
Post-translational Modifications: Phosphorylation at Ser-56 favors interaction with CALM1. Isoform 1 is methylated by CARM1 in immature thymocytes.
Subunit Structure: Interacts with CALM1.
Post-translational Modifications: Phosphorylation at Ser-56 favors interaction with CALM1. Isoform 1 is methylated by CARM1 in immature thymocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 80 kD
Predicted: 90 kDa
Predicted: 90 kDa
NCBI Official Full Name
cAMP-regulated phosphoprotein 21 isoform 2
NCBI Official Synonym Full Names
cAMP regulated phosphoprotein 21
NCBI Official Symbol
ARPP21
NCBI Official Synonym Symbols
RCS; TARPP; R3HDM3; ARPP-21
NCBI Protein Information
cAMP-regulated phosphoprotein 21
UniProt Protein Name
cAMP-regulated phosphoprotein 21
Protein Family
UniProt Gene Name
ARPP21
UniProt Synonym Gene Names
TARPP; ARPP-21
NCBI Description
This gene encodes a cAMP-regulated phosphoprotein. The encoded protein is enriched in the caudate nucleus and cerebellar cortex. A similar protein in mouse may be involved in regulating the effects of dopamine in the basal ganglia. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
Uniprot Description
Isoform 2 may act as a competitive inhibitor of calmodulin-dependent enzymes such as calcineurin in neurons.
Research Articles on ARPP21
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Product Notes
The ARPP21 arpp21 (Catalog #AAA9605327) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARPP21 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ARPP21 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the ARPP21 arpp21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARPP21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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