Western Blot (WB)
(Staining (1ug/ml) of Mouse Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat ARPC3 Polyclonal Antibody | anti-ARPC3 antibody
Goat anti-ARPC3 Antibody
Gene Names
ARPC3; ARC21; p21-Arc
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ARPC3; Polyclonal Antibody; Goat anti-ARPC3 Antibody; ARC21; p21-Arc; actin related protein 2/3 complex; subunit 3; 21kDa; ARP2/3 protein complex subunit p21; actin related protein 2/3 complex subunit 3; ARPC3 antibody; ARC21 antibody; p21-Arc antibody; 21kDa antibody; ARP2/3 protein complex subunit p21 antibody; actin related protein 2/3 complex subunit 3 antibody; anti-ARPC3 antibody
Host
Goat
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RQFMNKSLSGPGQ
Sequence Length
178
Applicable Applications for anti-ARPC3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Band observed just below 20kDa marker in Hela, Human Brain and Mouse Brain lysates (predicted MW of 20kDa according to NP_005710). Recommended for use at 1-3ug/ml.
Western Blot: Band observed just below 20kDa marker in Hela, Human Brain and Mouse Brain lysates (predicted MW of 20kDa according to NP_005710). Recommended for use at 1-3ug/ml.
Immunogen
Peptide with sequence C-RQFMNKSLSGPGQ, from the C Terminus of the protein sequence according to NP_005710.1.
Epitope
C Terminus
Note
Please note there are two hypothetical proteins called "similar to the Arp2/3 protein complex subunit p21-Arc" that are virtually identical (CAC14083, XP_208062).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
(Staining (1ug/ml) of Mouse Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (1ug/ml) of Mouse Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,547 Da
NCBI Official Synonym Full Names
actin related protein 2/3 complex subunit 3
NCBI Official Symbol
ARPC3
NCBI Official Synonym Symbols
ARC21; p21-Arc
NCBI Protein Information
actin-related protein 2/3 complex subunit 3
UniProt Protein Name
Actin-related protein 2/3 complex subunit 3
Protein Family
UniProt Gene Name
ARPC3
UniProt Synonym Gene Names
ARC21; p21-ARC
UniProt Entry Name
ARPC3_HUMAN
NCBI Description
This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been conserved through evolution and is implicated in the control of actin polymerization in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]
Uniprot Description
ARPC3: one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 12q24.11
Cellular Component: actin cytoskeleton; Arp2/3 protein complex; cytosol; focal adhesion; lamellipodium; membrane
Molecular Function: actin filament binding; protein binding; structural constituent of cytoskeleton
Biological Process: cell motility; ephrin receptor signaling pathway
Research Articles on ARPC3
Similar Products
Product Notes
The ARPC3 arpc3 (Catalog #AAA420461) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ARPC3 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ARPC3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Band observed just below 20kDa marker in Hela, Human Brain and Mouse Brain lysates (predicted MW of 20kDa according to NP_005710). Recommended for use at 1-3ug/ml. Researchers should empirically determine the suitability of the ARPC3 arpc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RQFMNKSLSG PGQ. It is sometimes possible for the material contained within the vial of "ARPC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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