Activity Data
(IL-1beta (Interleukin-1 beta) is a proinflammatory and immunoregulatory cytokine involved in a variety of cellular activities. It is produced by activated macrophages as a proprotein, and then proteolytically processed to an active form. It has been reported that IL-1beta-induced IL-6 production is mediated by both PI3K and IRAK4 in A549 cells. To detect the bioactivity of IL-1beta, A549 cells were seeded into 24-well plate at a density of 1x10^5cells/mL, and allowed to attach overnight before treated with or without certain concentrations (1ng/mL, 10ng/mL) of IL1-beta for 4h and IL-6 levels in the cell supernatant were determined by ELISA.IL-6 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL1-beta, the data was shown in Table 1 and Figure 1.)
Interleukin 1 Beta (IL1b) Active Protein | IL1b active protein
Active Interleukin 1 Beta (IL1b)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Activity Data
(IL-1beta (Interleukin-1 beta) is a proinflammatory and immunoregulatory cytokine involved in a variety of cellular activities. It is produced by activated macrophages as a proprotein, and then proteolytically processed to an active form. It has been reported that IL-1beta-induced IL-6 production is mediated by both PI3K and IRAK4 in A549 cells. To detect the bioactivity of IL-1beta, A549 cells were seeded into 24-well plate at a density of 1x10^5cells/mL, and allowed to attach overnight before treated with or without certain concentrations (1ng/mL, 10ng/mL) of IL1-beta for 4h and IL-6 levels in the cell supernatant were determined by ELISA.IL-6 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL1-beta, the data was shown in Table 1 and Figure 1.)
Activity Data
(IL-1beta (Interleukin-1 beta) is a proinflammatory and immunoregulatory cytokine involved in a variety of cellular activities. It is produced by activated macrophages as a proprotein, and then proteolytically processed to an active form. It has been reported that IL-1beta-induced IL-6 production is mediated by both PI3K and IRAK4 in A549 cells. To detect the bioactivity of IL-1beta, A549 cells were seeded into 24-well plate at a density of 1x10^5cells/mL, and allowed to attach overnight before treated with or without certain concentrations (1ng/mL, 10ng/mL) of IL1-beta for 4h and IL-6 levels in the cell supernatant were determined by ELISA.IL-6 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL1-beta, the data was shown in Table 1 and Figure 1.)
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Testing Data
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NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Uniprot Description
IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family.
Protein type: Cytokine
Chromosomal Location of Human Ortholog: 2q14.1
Cellular Component: cytosol; extracellular region; extracellular space
Molecular Function: cytokine activity; interleukin-1 receptor binding; protein domain specific binding
Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; apoptosis; cell-cell signaling; cytokine and chemokine mediated signaling pathway; embryo implantation; hyaluronan biosynthetic process; inflammatory response; lipopolysaccharide-mediated signaling pathway; MAPKKK cascade; negative regulation of cell proliferation; negative regulation of insulin receptor signaling pathway; negative regulation of lipid catabolic process; negative regulation of lipid metabolic process; negative regulation of MAP kinase activity; positive regulation of angiogenesis; positive regulation of fever; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of heterotypic cell-cell adhesion; positive regulation of interferon-gamma production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of JNK cascade; positive regulation of lipid catabolic process; positive regulation of membrane protein ectodomain proteolysis; positive regulation of mitosis; positive regulation of NF-kappaB import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of phagocytosis; positive regulation of prostaglandin secretion; positive regulation of protein amino acid phosphorylation; positive regulation of T cell mediated immunity; positive regulation of T cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; positive regulation of vascular endothelial growth factor receptor signaling pathway; protein kinase B signaling cascade; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of insulin secretion; regulation of nitric-oxide synthase activity; sequestering of triacylglycerol; signal transduction
Disease: Gastric Cancer, Hereditary Diffuse
Research Articles on IL1b
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Product Notes
The IL1b il1b (Catalog #AAA2086080) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Interleukin 1 Beta (IL1b) can be used in a range of immunoassay formats including, but not limited to, Cell culture, Activity Assays. Researchers should empirically determine the suitability of the IL1b il1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 1 Beta (IL1b), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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