Rabbit anti-Human ARL3 Polyclonal Antibody | anti-ARL3 antibody

ARL3, NT (ADP-ribosylation Factor-like Protein 3, ARFL3) (AP)

Cat. Num. AAA6464032

• Gene Names: ARL3; ARFL3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: ARL3; Polyclonal Antibody; NT (ADP-ribosylation Factor-like Protein 3; ARFL3) (AP); anti-ARL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ARL3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ARL3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human ARL3 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-hARL3-M1 Pab in A2058 cell line lysate (35ug/lane). hARL3-M1(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-ARL3 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

1. Cavenagh, M.M., et al., J. Biol. Chem. 269(29):18937-18942 (1994). 2. Adams, M.D., et al., Nature 377(6547 Suppl), 3-174 (1995). 3. Kim, H.S., Cytogenet. Cell Genet. 83(3-4), 246 (1998). 4. Wistow, G., et al., Mol. Vis. 8, 196-204 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4757774
• NCBI GeneID: 403
• NCBI Accession #: NP_004302
• NCBI GenBank Nucleotide #: NM_004311.3
• UniProt Accession #: P36405; Q53X83; Q5JSM2; B2R6C7
• Molecular Weight: 23.0 kDa (206aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ADP-ribosylation factor-like protein 3
• NCBI Official Synonym Full Names: ADP ribosylation factor like GTPase 3
• NCBI Official Symbol: ARL3
• NCBI Official Synonym Symbols: ARFL3
• NCBI Protein Information: ADP-ribosylation factor-like protein 3
• UniProt Protein Name: ADP-ribosylation factor-like protein 3
• Protein Family: ADP-ribosylation factor-like protein
• UniProt Gene Name: ARL3
• UniProt Synonym Gene Names: ARFL3
• UniProt Entry Name: ARL3_HUMAN

NCBI Description

ADP-ribosylation factor-like 3 is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. [provided by RefSeq, Jul 2008]

Uniprot Description

ARL3: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase- activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit. Belongs to the small GTPase superfamily. Arf family.

Protein type: Cell cycle regulation; Cytoskeletal; G protein, monomeric, ARF; G protein, monomeric

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: Golgi membrane; Golgi apparatus; centrosome; cytoplasmic microtubule; spindle microtubule; midbody; nucleus; photoreceptor connecting cilium

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; microtubule binding; metal ion binding

Biological Process: photoreceptor cell development; protein transport; smoothened signaling pathway; metabolic process; organelle organization and biogenesis; small GTPase mediated signal transduction; intraflagellar transport; cytokinesis; post-Golgi vesicle-mediated transport; kidney development